Influence of Salix Babylonica Extract in Combination or not with Increasing Levels of Minerals Mixture on in Vitro Rumen Gas Production Kinetics of a Total Mixed Ration

Figures & data

Table 1. Ingredient and chemical composition of the total mixed ration.

	Concentrate	Corn silage
Ingredients, g/kg DM
Soybean meal	220
Alfalfa hay	195
Sorghum grain	550
Fish meal	35
Chemical composition, g/kg DM
Crude protein	147	106
Ether extract	120	85
Neutral detergent fibre	160	445
Acid detergent fibre	28	111
Acid detergent lignin	8	18

n=3; standard deviation=0.43.

Table 2. In vitro rumen gas kinetics and cumulative gas production after 72 h of incubation as affected by the combination of Salix babylonica extract doses and minerals levels.

SB dosage,	MM, DM	GP parameters			In vitro GP, mL/g DM
mL/g DM	g/100 g	b, mL/g DM	c, /h	L, h	GP2	GP4	GP6	GP8	GP10	GP12	GP24	GP36	GP48	GP72
0	0	204	0.052	1.83	20.2	38.4	54.8	69.6	83.0	95.0	145.8	173.0	187.6	199.6
0.5	212	0.046	2.14	18.8	35.9	51.5	65.7	78.6	90.4	142.2	171.8	188.9	204.4
1.5	217	0.053	1.42	21.9	41.6	59.3	75.2	89.5	102.4	156.5	185.1	200.3	212.5
2.5	222	0.054	1.23	22.6	42.9	61.1	77.5	92.2	105.4	160.8	189.9	205.2	217.5
0.6	0	210	0.053	1.88	21.1	40.0	57.0	72.4	86.1	98.5	150.8	178.6	193.3	205.4
0.5	211	0.056	2.01	22.5	42.6	60.5	76.5	90.8	103.6	156.2	183.0	196.7	207.4
1.5	215	0.057	1.68	23.0	43.6	62.0	78.4	93.0	106.1	159.9	187.2	201.1	211.6
2.5	215	0.057	1.83	23.2	44.0	62.5	79.0	93.7	106.8	160.5	187.5	201.1	211.4
1.2	0	214	0.057	1.89	22.8	43.2	61.4	77.6	92.1	105.1	158.4	185.5	199.2	209.8
0.5	234	0.068	2.05	30.0	56.2	79.0	98.9	116.2	131.3	188.9	214.2	225.5	232.7
1.5	233	0.069	2.36	29.9	56.0	78.6	98.4	115.6	130.6	187.7	212.8	224.0	231.1
2.5	233	0.059	2.30	25.8	48.7	69.1	87.1	103.2	117.4	175.3	204.0	218.4	229.3
1.8	0	242	0.081	2.97	36.0	66.7	92.8	115.0	133.9	150.0	207.0	228.7	236.9	241.3
0.5	251	0.068	2.55	31.7	59.3	83.4	104.4	122.8	138.8	200.4	227.9	240.3	248.5
1.5	239	0.073	3.13	32.3	60.2	84.3	105.2	123.2	138.8	197.0	221.4	231.8	238.0
2.5	242	0.067	2.83	30.5	57.2	80.5	100.9	118.7	134.2	193.9	220.6	232.5	240.2
Pooled SEM		10.0	2.377	0.002	2.118	3.76	2.36	3.82	6.181	8.45	9.65	7.998	2.8319	2.6458
P
SB main effect		0.0250	<0.0001	<0.001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
Linear		0.0060	<0.0001	<0.001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
Quadratic		0.3740	0.0019	0.1210	0.0336	0.0153	0.0151	0.0147	0.0143	0.0138	0.0133	0.0098	0.0067	0.0048
MM main effect		0.0740	0.0130	0.3860	0.8573	0.4353	0.4104	0.3832	0.3560	0.3270	0.2982	0.1465	0.0643	0.0325
Linear		0.1160	0.0147	0.8710	0.4965	0.5330	0.4762	0.4218	0.3718	0.3249	0.2822	0.1096	0.0435	0.0225
Quadratic		0.0380	0.1299	0.2610	0.6047	0.1403	0.1343	0.1286	0.1236	0.1187	0.1142	0.0961	0.0912	0.0961
Interaction SB×MM		0.2840	0.2319	0.0101	0.1410	0.0142	0.0151	0.0161	0.0171	0.0183	0.0197	0.0321	0.0557	0.0919

SB, Salix babylonica; MM, minerals mixture; DM, dry matter; GP, gas production; b, asymptotic gas production; c, rate of gas production; L, initial delay before gas production begins.

Table 3. In vitro rumen fermentation profile as affected by the combination of Salix babylonica extract doses and minerals levels.

SB dosage, mL/g DM	MM, g/100 g DM	pH	DMD, mg/g DM	OMD, %	ME, MJ/kg DM	PF24, mg DMD:mL gas	GY24, mL gas/g DMD	SCFA, mmol/g DM	MCP, mg/g DM
0	0	6.51	732.46	45.20	6.68	5.03	199.06	3.22	411.69
0.5	6.50	743.87	44.55	6.58	5.24	191.08	3.13	431.14
	1.5	6.47	736.51	47.10	6.97	4.54	258.32	3.45	377.88
2.5	6.50	758.62	47.86	7.09	4.72	211.94	3.55	404.92
0.6	0	6.53	762.14	46.08	6.82	5.06	197.88	3.33	430.37
0.5	6.49	755.78	47.04	6.96	4.87	206.89	3.45	412.23
1.5	6.48	740.21	47.71	7.07	4.63	216.02	3.53	388.38
2.5	6.49	749.76	47.81	7.08	4.67	214.05	3.54	396.68
1.2	0	6.48	752.83	47.43	7.02	4.75	210.40	3.49	404.41
0.5	6.47	742.38	52.86	7.85	3.95	254.64	4.17	326.84
1.5	6.49	748.56	52.65	7.82	4.01	250.99	4.15	335.62
2.5	6.47	767.31	50.44	7.48	4.41	228.31	3.87	381.70
1.8	0	6.48	756.86	56.07	8.35	3.66	273.50	4.57	301.54
0.5	6.46	753.96	54.90	8.17	3.78	265.80	4.43	313.17
1.5	6.47	743.01	54.30	8.08	3.77	265.21	4.35	309.61
2.5	6.48	733.63	53.75	7.99	3.79	264.45	4.28	307.00
Pooled SEM		0.006	3.637	0.533	0.082	0.081	6.697	0.067	8.119
P value
SB main effect		0.0250	0.2017	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
Linear		0.0060	0.4369	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001
Quadratic		0.3741	0.0471	0.0098	0.0098	0.0076	0.0085	0.0102	0.0019
MM main effect		0.0736	0.2128	0.1471	0.1541	0.0177	0.0484	0.1465	0.0465
Linear		0.1159	0.9602	0.1099	0.1116	0.0302	0.1843	0.1080	0.1580
Quadratic		0.0381	0.0541	0.0966	0.1021	0.0172	0.0170	0.0970	0.0144
Interaction SB×MM		0.2841	0.0419	0.0322	0.0335	0.0413	0.1409	0.0324	0.1024

SB, Salix babylonica; MM, minerals mixture; DM, dry matter; DMD, DM degradability; OMD, in vitro organic matter digestibility; ME, metabolizable energy; PF₂₄, partitioning factor at 24 h of incubation; GY₂₄, gas yield at 24 h of incubation; SCFA, short chain fatty acids; MCP, microbial crude protein production.