Feasibility Study on the FAO Chicken Microsatellite Panel to Assess Genetic Variability in the Turkey (Meleagris Gallopavo)

Figures & data

Table 1. Polymerase chain reaction conditions, multiplex groups, number and allele size of chicken Food and Agriculture Organization microsatellites identified in the turkey.

Locus	PCR condition		Multiplex group^°	Alleles, n	Allele size
	T, °C	Taq protocol			NI	BR	EU
ADL0112	58	MasterTaq Kit	-	5	127, 131, 139	127, 129, 131	131, 133
ADL0268	48	AmpliTaq Gold^®	Mix 1	6	97, 101, 109, 111	95, 97, 101, 109, 111	97, 109, 111, 113
ADL0278	48	AmpliTaq Gold^®	Mix 1	5	113, 119	113, 119, 121	113, 117, 119, 125
	58	MasterTaq Kit
LEI0094	60	MasterTaq Kit	Mix 1	3	267	261, 281	261, 267
LEI0166	60	MasterTaq Kit	No amplification	-
LEI0192	58	MasterTaq Kit	-	1	257	257	257
LEI0234	58	MasterTaq Kit	-	3	220, 304	220, 304, 308	220, 304
MCW0014	55	AmpliTaq Gold^®	-	3	193	179, 193	179, 193
MCW0016	60	MasterTaq Kit	-	2	132, 144	132	132
MCW0020	60	MasterTaq Kit	No amplification	-
MCW0034	58	MasterTaq Kit	Mix 2	3	222, 230, 234	234	234
MCW0037	58	MasterTaq Kit	-	3	155	151, 155, 157	155
MCW0067	58	MasterTaq Kit	-	5	173, 175, 177, 181, 183	175, 177, 181	175, 177, 181
MCW0069	55	AmpliTaq Gold^®	Mix 2	2	159	159, 165	159
MCW0078	55	AmpliTaq Gold^®	No amplification	-
MCW0080	60	MasterTaq Kit	No amplification	-
MCW0081	58	MasterTaq Kit	Mix 2	3	110, 114	114, 118	110, 114
MCW0098	55	AmpliTaq Gold^®	-	2	227	227, 233	227
MCW0103	58	MasterTaq Kit	-	3	268	268, 282	268, 272
MCW0104	52	AmpliTaq Gold^®	No amplification	-
MCW0111	55	AmpliTaq Gold^®	-	2	97, 99	97, 99	99
MCW0123	53	AmpliTaq Gold^®	No amplification	-
MCW0165	58	MasterTaq Kit	-	1	116	116	116
MCW0183	60	MasterTaq Kit	-	2	287	283, 287	287
MCW0206	55	AmpliTaq Gold^®	-	1	223	223	223
MCW0216	58	MasterTaq Kit	Mix 1	2	145, 155	145, 155	145, 155
MCW0222	55	AmpliTaq Gold^®	Mix 2	3	222, 224, 226	222, 224, 226	222
MCW0248	60	MasterTaq Kit	No amplification	-
MCW0284	58	MasterTaq Kit	No amplification	-
MCW0295	55	AmpliTaq Gold^®	Mix 2	3	90, 92, 102	90, 102	90
MCW0330	58	MasterTaq Kit	No amplification	-

PCR, polymerase chain reaction; NI, Nero d’Italia; BR, Brianzolo; EU, Colli Euganei. Information about the size of the fragments and PCR primers in the chicken are reported in FAO Guidelines (2011). Genomic DNA obtained from different breeds was analysed with two protocols (MasterTaq Kit: 5PRIME, Gaithersburg, MD, USA; AmpliTaq Gold^®: Life Technologies, Carlsbad, CA, USA).

°Column shows the multiplex groups identified in turkeys out of the 7 proposed by FAO for the chicken, the microsatellites amplified in single reaction (-) and microsatellites with no amplification.

Table 2. Observed and expected heterozygosity and polymorphism information content values calculated by locus in different turkey breeds.

Locus	Ho			He			PIC
	NI	BR	EU	NI	BR	EU	NI	BR	EU
ADL0112	0.333	0.143	0.200	0.733	0.385	0.467	0.535	0.325	0.332
ADL0268	0.500	0.444	0.667	0.742	0.614	0.561	0.622	0.544	0.476
ADL0278	0.500	0.667	0.500	0.571	0.621	0.767	0.375	0.477	0.667
LEI0094	0	0	0.250	0	0.533	0.536	0	0.346	0.359
LEI0234	0.500	0.500	0.750	0.500	0.714	0.536	0.305	0.555	0.359
MCW0014	0	0	0.333	0	0.303	0.333	0	0.239	0.239
MCW0016	0.500	0	0	0.429	0	0	0.305	0	0
MCW0034	0.250	0	0	0.750	0	0	0.582	0	0
MCW0037	0	0.222	0	0	0.627	0	0	0.527	0
MCW0067	0.714	0.625	0.667	0.791	0.700	0.621	0.689	0.582	0.477
MCW0069	0	0.167	0	0	0.167	0	0	0.141	0
MCW0081	0	0.125	0	0.533	0.325	0.264	0.346	0.258	0.215
MCW0098	0	0.750	0	0	0.536	0	0	0.359	0
MCW0103	0	0.500	1.000	0	0.500	0.571	0	0.305	0.375
MCW0111	0.571	0.308	0	0.440	0.492	0	0.325	0.361	0
MCW0183	0	0.200	0	0	0.189	0	0	0.164	0
MCW0216	0.100	0.214	0.091	0.100	0.198	0.368	0.090	0.173	0.290
MCW0222	0.333	0.500	0	0.545	0.607	0	0.449	0.468	0
MCW0295	0.429	0.143	0	0.484	0.143	0	0.406	0.124	0
Mean	0.210	0.250	0.203	0.301	0.348	0.228	0.265	0.313	0.199

Ho, observed heterozygosity; He, expected heterozygosity; PIC, polymorphism information content; NI, Nero d’Italia; BR, Brianzolo; EU, Colli Euganei.

FAO, 2011. Molecular genetic characterization of animal genetic resources. Food and Agriculture Organization of the United Nations Publ., Rome, Italy. Available from: http://www.fao.org/docrep/014/i2413e/i2413e00.pdf

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