Effect of Confinement and Starvation on Stress Parameters in the American Lobster (Homarus Americanus)

Figures & data

Figure 1. Map of the route taken by the lobsters from Canada to Italy.

Figure 2. Variation of glucose concentrations in hemolymph of H. americanus during the experiment. Values are expressed as means±standard error.

Figure 3. Variation of protein concentrations in hemolymph of H. americanus during the experiment. Values are expressed as means±standard error.

Figure 4. Variation of total haemocyte count concentrations in hemolymph of H. americanus during the experiment. Values are expressed as means±standard error.

Figure 5. Proximate muscle composition of H. americanus during the trial.

Figure 6. Time course with anti-actin detection.

Figure 7. Time course of total proteins in Coomassie staining.

Table 1. Changes in water quality parameters during the trial.

Variables	Storage time, weeks					SE (17 df)
	T0	1^st	2^nd	3^rd	4^th
Temperature, °C	8.5	8.3	7.8	8.1	7.5	0.44
pH	7.5	7.6	7.8	7.8	7.7	0.007
NH4-N, mg/L	1.6	1.7	1.3	0.6	1.1	0.033
NO2-N, mg/L	0.3	0.4^b	1.1^b	2.1^b	4.7^a	0.45
NO3-N, mg/L	<5	<5	<5	<5	<5	-
O2, mg/L	6.4^B	6.7^B	7.3^A	7.1^A	6.8^B	1.70
O2, % sat	72.0^B	73.8^B	81.3^A	83.3^A	77.4^B	215.4
Salinity, ‰	33.0	33.0	33.0	33.0	33.1	0.25

SE, standard error of variance; df, degree of freedom.

^a,bP<0.05

^A,BP<0.01. Different letters in the same row denote significant differences among treatments.

Table 2. Variation of body weights of lobsters during the experiment compared to the initial value.

Variable	Tank	T0	End of the trial (weeks)
Body weight, g	1	534±26.0	540±24.7 (1^st)
2	546±38.0	553±39.7 (2^nd)
3	528±43.6	541±39.9 (3^rd)
4	570±39.9	576±39.2 (4^th)