A Sensitive and Accurate Method to Simultaneously Measure Uric Acid and Creatinine in Human Saliva by Using LC–MS/MS

Abstract

Aim: To establish a method to simultaneously measure uric acid (UA) and creatinine (Cr) in human saliva. Materials & methods: By using HPLC–MS/MS, we developed and validated a fast, sensitive and accurate method to simultaneously determine UA and Cr in human saliva. The determination range for Cr and UA is of 10–5000 ng/ml with the R² for both calibration curves over 0.999. The accuracy, precision and recovery of Cr and UA were all acceptable. By using the established method, the Cr and UA levels in saliva from 28 healthy volunteers were measured as 2.9 ± 0.8 µM and 46.8 ± 18.2 µM, respectively. Conclusion: This method can simultaneously determine Cr and UA in saliva for clinical and translational study.

Keywords::

• creatinine
• LC–MS/MS
• saliva
• uric acid

Author contributions

JY Liu designed the research. XY Liu, Y Luo, CY Zhou and A Peng conducted the research. XY Liu, Y Luo and JY Liu analyzed the data. JY Liu wrote and finalized the paper. All the authors critically reviewed and approved the paper.

Financial and competing interests disclosure

This study was supported by National Natural Science Foundation of China (NSFC 81470588) for JY Liu and the Fundamental Research Funds for the Central Universities (2016KJ048) for Y Luo. The authors conducted this study independently. The funding organization played no role in the study design, data collection, analysis and interpretation, manuscript preparation, or in the decision to submit the report for publication. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.

No writing assistance was utilized in the production of this manuscript.

Ethical conduct of research

The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human investigation.

Acknowledgements

The authors would like to thank all the volunteers for their enthusiasm in participation and the great contribution to this study.