1,670
Views
4
CrossRef citations to date
0
Altmetric
Research Article

Barberry's (Berberis integerrima) ingredients suppress T-cell response and shift immune responses toward Th2: an in vitro study

, &
Article: FSO49 | Published online: 15 Jul 2015

Figures & data

Figure 1. The values (mean ± standard deviation) of proliferation assay of lypopolysaccharide/phytohemagglutinin/un-stimulated spelnocytes treated with various concentrations of Berberis integerrima alcoholic extract.

1–1000 μg/ml of extracts reduced PHA stimulated splenocytes (as T cells) proliferation and enhanced proliferation of LPS stimulated (as B cells) or unstimulated splenocytes. Significant differences are designated as *p < 0.05.

LPS: Lypopolysaccharide; PHA: Phytohemagglutinin.

Figure 1.  The values (mean ± standard deviation) of proliferation assay of lypopolysaccharide/phytohemagglutinin/un-stimulated spelnocytes treated with various concentrations of Berberis integerrima alcoholic extract.1–1000 μg/ml of extracts reduced PHA stimulated splenocytes (as T cells) proliferation and enhanced proliferation of LPS stimulated (as B cells) or unstimulated splenocytes. Significant differences are designated as *p < 0.05.LPS: Lypopolysaccharide; PHA: Phytohemagglutinin.

Figure 2. The values (mean ± standard deviation) of proliferation assay of lypopolysaccharide/phytohemagglutinin/un-stimulated spelnocytes treated with various concentrations of Berberis integerrima aqueous extract.

0.01–1000 μg/ml of extracts reduced PHA stimulated splenocytes (as T cells) proliferation. Significant differences are designated as *p < 0.05.

LPS: Lypopolysaccharide; PHA: Phytohemagglutinin.

Figure 2.  The values (mean ± standard deviation) of proliferation assay of lypopolysaccharide/phytohemagglutinin/un-stimulated spelnocytes treated with various concentrations of Berberis integerrima aqueous extract.0.01–1000 μg/ml of extracts reduced PHA stimulated splenocytes (as T cells) proliferation. Significant differences are designated as *p < 0.05.LPS: Lypopolysaccharide; PHA: Phytohemagglutinin.

Figure 3. IFN-γ production in splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.

Significant differences were designated as *p < 0.05.

SD: Standard deviation.

Figure 3.  IFN-γ production in splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.Significant differences were designated as *p < 0.05.SD: Standard deviation.

Figure 4. IL-4 production splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.

Significant differences were designated as *p < 0.05.

SD: Standard deviation.

Figure 4.  IL-4 production splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.Significant differences were designated as *p < 0.05.SD: Standard deviation.

Figure 5. IL-10 production splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.

Significant differences were designated as *p < 0.05.

SD: Standard deviation.

Figure 5.  IL-10 production splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.Significant differences were designated as *p < 0.05.SD: Standard deviation.

Figure 6. TGF-β production of splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.

Significant differences were designated as *p < 0.05.

SD: Standard deviation.

Figure 6.  TGF-β production of splenocytes treated with various concentrations of Berberis integerrima (A) alcoholic and (B) aqueous extracts.Significant differences were designated as *p < 0.05.SD: Standard deviation.