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Future Science OA Volume 1, 2015 - Issue 4
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Preliminary Communication

Conducting Polymer-Based Multilayer Films for Instructive Biomaterial Coatings

John G Hardy1 J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL32611, USA;2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USACorrespondence[email protected]
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,
Hetian Li2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USAView further author information
,
Jacqueline K Chow2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USAView further author information
,
Sydney A Geissler1 J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL32611, USA;2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USAView further author information
,
Austin B McElroy2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USAView further author information
,
Lindsey Nguy2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USAView further author information
,
Derek S Hernandez2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USAView further author information
&
Christine E Schmidt1 J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL32611, USA;2 Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX78712, USACorrespondence[email protected]
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Article: FSO79 | Received 30 Jul 2015, Accepted 04 Sep 2015, Published online: 02 Nov 2015

Figures & data

Figure 1.  Molecules employed in this study.

CHI: Chitosan; GEN: Genipin; PEDOT: Poly(3,4-ethylenedioxythiophene); PEDOT-S: Sulfonated PEDOT derivative; PSS: Polystyrenesulfonate.

Figure 1.  Molecules employed in this study.CHI: Chitosan; GEN: Genipin; PEDOT: Poly(3,4-ethylenedioxythiophene); PEDOT-S: Sulfonated PEDOT derivative; PSS: Polystyrenesulfonate.

Figure 2.  Adhesion and growth of human dermal fibroblasts on various surfaces up to 4 days in culture.

(A) Tissue culture plate controls. (B) PEDOT-PSS-based multilayer films. (C) PEDOT-S-based multilayer films. Cells were stained with a LIVE/DEAD® Viability/Cytotoxicity Kit, live cells were green and dead cells were red. Scale bars represent 250 µm. (D) Cell viability after 4 days in culture as determined with a LIVE/DEAD® Viability/Cytotoxicity Kit. (E) Number of cells adhered to the substrates after 2 days (grey bars) or 4 days (black bars) as estimated by the AlamarBlue® assay. Error bars represent standard deviations (n = 3).

Figure 2.  Adhesion and growth of human dermal fibroblasts on various surfaces up to 4 days in culture.(A) Tissue culture plate controls. (B) PEDOT-PSS-based multilayer films. (C) PEDOT-S-based multilayer films. Cells were stained with a LIVE/DEAD® Viability/Cytotoxicity Kit, live cells were green and dead cells were red. Scale bars represent 250 µm. (D) Cell viability after 4 days in culture as determined with a LIVE/DEAD® Viability/Cytotoxicity Kit. (E) Number of cells adhered to the substrates after 2 days (grey bars) or 4 days (black bars) as estimated by the AlamarBlue® assay. Error bars represent standard deviations (n = 3).

Figure 3.  (A) Experimental setup for electrical stimulation of PEDOT-based films (not to scale). (B) Cell alignment and length assessment.

CE: Counter electrode; CT: Copper tape; PCW: Polycarbonate well; RE: Reference electrode; WE: Working electrode.

Adapted with permission from [Citation33] © 2015 Hardy JG et al.

Figure 3.  (A) Experimental setup for electrical stimulation of PEDOT-based films (not to scale). (B) Cell alignment and length assessment.CE: Counter electrode; CT: Copper tape; PCW: Polycarbonate well; RE: Reference electrode; WE: Working electrode.Adapted with permission from [Citation33] © 2015 Hardy JG et al.

Figure 4.  Analysis of the morphology of human dermal fibroblasts on various surfaces with optional electrical stimulation (applied in line with the dipping direction and horizontally relative to the images presented).

(A) PEDOT-PSS-based multilayer films without electrical stimulation, PEDOT-PSS(-). (B) PEDOT-PSS-based multilayer films with electrical stimulation, PEDOT-PSS(+). (C) PEDOT-S-based multilayer films without electrical stimulation, PEDOT-S(-). (D) PEDOT-S-based multilayer films with electrical stimulation PEDOT-S(+). DAPI-stained nuclei are blue and Alexa Fluor® 488-stained actin is green. Scale bars represent 200 µm. (E) Assessment of cell alignment. (F) Assessment of cell length. Error bars represent standard errors of the mean (n = 150 or more).

*p ≤ 0.01, **p < 0.001.

Figure 4.  Analysis of the morphology of human dermal fibroblasts on various surfaces with optional electrical stimulation (applied in line with the dipping direction and horizontally relative to the images presented).(A) PEDOT-PSS-based multilayer films without electrical stimulation, PEDOT-PSS(-). (B) PEDOT-PSS-based multilayer films with electrical stimulation, PEDOT-PSS(+). (C) PEDOT-S-based multilayer films without electrical stimulation, PEDOT-S(-). (D) PEDOT-S-based multilayer films with electrical stimulation PEDOT-S(+). DAPI-stained nuclei are blue and Alexa Fluor® 488-stained actin is green. Scale bars represent 200 µm. (E) Assessment of cell alignment. (F) Assessment of cell length. Error bars represent standard errors of the mean (n = 150 or more).*p ≤ 0.01, **p < 0.001.

Table 1.  Surface properties of the films.

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