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Research Article

‘What did I do Wrong?’ an Empirical Evaluation of Sample Preparation Methodologies in Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging

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Article: FSO362 | Received 02 Oct 2018, Accepted 29 Oct 2018, Published online: 07 Feb 2019

Figures & data

Figure 1. Mouse lymph node: correctly prepared.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 1.  Mouse lymph node: correctly prepared.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 2. Human spinal cord.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 2.  Human spinal cord.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 3. Lamb liver.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 3.  Lamb liver.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 4. PK15 cell line monolayer.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 4.  PK15 cell line monolayer.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 5. Mouse heart 1.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 5.  Mouse heart 1.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 6. Mouse kidney.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 6.  Mouse kidney.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 7. Mouse liver 1.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 7.  Mouse liver 1.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 8. Mouse spinal cord.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 8.  Mouse spinal cord.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 9. Human brain.

Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.

Figure 9.  Human brain.Green outlines indicate the borders of actual tissue sections relative to their position on the slide. Blue boxes show the area that was actually imaged.
Supplemental material

supplementary data.docx

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