Figures & data
Figure 1. Model for Atg1 complex assembly. Left, the Atg1 EAT domain dimer is partially melted in the absence of Atg13. Middle, the EAT domain become rigidified when Atg13 binds to it. The parts of Atg13 that directly bind to Atg1 also become ordered. The equilibrium strongly favors Atg1-Atg13 complex formation, at least when Atg13 is dephosphorylated. Right, the Atg1-Atg13 subcomplex binds weakly to the tips of the Atg17-Atg31-Atg29 double crescent. A 4:4:2:2:2 complex of Atg1-Atg13-Atg17-Atg31-Atg29 is shown for illustration purposes, but in solution the hydrodynamic data suggest a mixture of 2:2:2:2:2 and 4:4:4:4:4 species.
