806
Views
0
CrossRef citations to date
0
Altmetric
SHORT COMMUNICATION

Light modulates the root tip excision induced lateral root formation in tomato

, &
Article: e970098 | Received 11 Jun 2014, Accepted 02 Jul 2014, Published online: 22 Dec 2014

Figures & data

Figure 1. Excision induced lateral root formation in tomato. (A) The root tips were excised from the seedlings at 4 DAG and seedlings were photographed at 7 DAG. (B) The root tips were excised either at 2 DAG or 4 DAG and number of lateral LRF was counted at 7 DAG. The control refers to intact seedlings. (n ≥ 10 ± SE) (C) The root tips were excised at 2 DAG from DR5::GUS transgenic seedlings. The roots were stained for GUS activity 24 h post-excision. (i) Intact roots at 2 DAG (ii) Root tip-excised roots after 24 h (iii) Intact seedling root at 3 DAG.

Figure 1. Excision induced lateral root formation in tomato. (A) The root tips were excised from the seedlings at 4 DAG and seedlings were photographed at 7 DAG. (B) The root tips were excised either at 2 DAG or 4 DAG and number of lateral LRF was counted at 7 DAG. The control refers to intact seedlings. (n ≥ 10 ± SE) (C) The root tips were excised at 2 DAG from DR5::GUS transgenic seedlings. The roots were stained for GUS activity 24 h post-excision. (i) Intact roots at 2 DAG (ii) Root tip-excised roots after 24 h (iii) Intact seedling root at 3 DAG.

Figure 2. Effect of ethylene and light on LRF. (A) The root tips were excised at 2 DAG and at the same time 20 μM ACC was supplemented to the germination boxes. The LRF was counted at 7 DAG. (n ≥ 10 ± SE) (B) The cotyledons or whole shoot was excised from seedlings at 2 DAG either with or without root tip excision. The LRF was counted at 7 DAG. The seedling with intact roots served as a control. (n ≥ 10 ± SE) (C) Effect of red (R), or far red (FR), and white light (WL) was examined after excision of root tips at 2 DAG or 4 DAG. The LRF was counted at 7 DAG. (n ≥ 10 ± SE).

Figure 2. Effect of ethylene and light on LRF. (A) The root tips were excised at 2 DAG and at the same time 20 μM ACC was supplemented to the germination boxes. The LRF was counted at 7 DAG. (n ≥ 10 ± SE) (B) The cotyledons or whole shoot was excised from seedlings at 2 DAG either with or without root tip excision. The LRF was counted at 7 DAG. The seedling with intact roots served as a control. (n ≥ 10 ± SE) (C) Effect of red (R), or far red (FR), and white light (WL) was examined after excision of root tips at 2 DAG or 4 DAG. The LRF was counted at 7 DAG. (n ≥ 10 ± SE).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.