Figures & data
Table 1. Summary of Documented Accounts of Studies Observing Interference when Delivering Multi-Valent DNA Vaccines
Figure 1. Multi-head device. (A) CAD concept drawing of multi-head hand piece. (B) Working prototype of 4 × 4 array with 1.5 mm spacing. (C) Close up of CAD concept disposable 2-head array (m2SEP). (D) Close up of CAD concept 4-disposable head array (m4SEP). (E) Wireless device in base station with Pocket PC displaying the user interface. (F) The m4SEP in use in a guinea pig model.
Figure 2. Overlap of reporter gene expression can be overcome by using the multi-head EP device. GFP and RFP reporter gene expression is used as a surrogate for visualizing the spatial separation of DNA plasmid vaccines. (A) Reporter gene plasmid (GFP and RFP) was delivered using the dual-head EP (m2SEP) device to spatially separate the 2 plasmids. (B) Reporter gene plasmid (GFP and RFP) was delivered using the quad-head EP (m4SEP) device to spatially separate the 2 plasmids. (C) GFP and RFP plasmid were mixed to mimic a cocktail vaccine and delivered using the single head EP device. (D) Intradermal injections of RFP and GFP plasmid were intentionally performed in close proximity of each other. The overlap of signal can be seen in the yellow band section.
Table 2. Average Antibody (PRNT50 GMT) Responses to DNA Vaccines Delivered by Multi-Head Electroporation Following 3 Vaccinations
Figure 3. Improved antibody titers are generated through the use of higher doses facilitated by delivery with the multi-head EP device. Higher magnitude antibody titers are generated in guinea pigs immunized with higher doses of influenza plasmids facilitated by the use of the multi-head EP (m4SEP) device. Endpoint titers for ELISA against H5HA delivered either as a single 25 μg dose with the SEP or as a 100 μg dose with the m4SEP.
