Study on development of Vipera lebetina snake anti-venom in chicken egg yolk for passive immunization

Figures & data

Figure 1. The Ouchterlony's double diffusion profile of egg yolk antibodies. middle well: 30 μg venom, wells no 1: 30 μg of crud egg yolk of test group, wells no 2: 30 μg of crud egg yolk of control group, wells no 3: 30 μg of purified fraction 1 of egg yolk from test group.

Figure 2. Fractionation of crud egg yolk by sephadex G-50. Fraction 1: Tube numbers 4–21 and Fraction 2: Tube numbers 29–33.

Figure 3. The SDS_PAGE pattern of the proteins of egg yolk of test group before and after purification. Standards (protein Mw marker), Egg yolk (Crud egg yolk), F₁ (Fraction 1), F₂ (Fraction 2).

Table 1. Vipera lebetina snake venom neutralization potency of chicken egg yolk antibody Fractions 1 (sample from the pooled first peak) and 2 (sample from the pooled second peak)

Animals Group*	Vipera lebetina Venom^+ Amount (LD50^#)	Chicken egg yolk antibody 0.75 gr/mL	IgY^! antivenom Volume (mL)	Horse^∼ antivenom Volume (mL)	Saline	Dead no.	% Dead	Neutralization potency (LD50/mL)
1	5	Fraction 1 (F1)	0.53			4	100	6
2			0.66			4	100
3			0.83			0	0
4			1.04			0	0
5			1.30			0	0
6	5	Fraction 2 (F2)	1.60			4	100	2
7			2.00			4	100
8			2.50			4	100
9			3.12			0	0
10			3.90			0	0
11	5			0.06		4	100	50
12				0.08		4	100
13				0.10		0	0
14				0.12		0	0
15				0.16		0	0
16	5				2.00	4	100	0
17					2.00	4	100
18					2.00	4	100
19					2.00	4	100
20					2.00	4	100

*Four mice's in each group, # Lethal dose 50%, + Standard Vipera lebetina snake venom with identified lethal activity, ! Chicken egg yolk antibody, ∼ Razi's Institute commercial horse antivenom with Vipera lebetina venom neutralization potency 50 LD₅₀/mL.