Figures & data
Figure 1. A map of Varanasi city showing collection spots of water samples. 1-Ganga river (Dhashaswamedh ghat), 2-Varuna river (Chowka ghat), 3-Varuna river (Dhobi ghat).
Figure 2. Representative HPLC chromatograms showing elution profile of: (A) standard (4-NP), (B) sample (Dashaswamedh ghat) and (C) standard + sample. Chromatographic conditions: volume: 20μL, column: C18 reverse phase [Luna (2) 150 X 45 mm i.d, 5μm], mobile phase (A) water and (B) acetonitrile, run time: 40 min, flow rate: 1ml/min, detection: UV 280 nm. In Y-axis, scales are not the same.
![Figure 2. Representative HPLC chromatograms showing elution profile of: (A) standard (4-NP), (B) sample (Dashaswamedh ghat) and (C) standard + sample. Chromatographic conditions: volume: 20μL, column: C18 reverse phase [Luna (2) 150 X 45 mm i.d, 5μm], mobile phase (A) water and (B) acetonitrile, run time: 40 min, flow rate: 1ml/min, detection: UV 280 nm. In Y-axis, scales are not the same.](/cms/asset/d13c0e6b-b3ff-4d9e-9a81-b4cac0d6c41e/kend_a_981442_f0002_b.gif)
Figure 3. Representative HPLC chromatograms showing 4-NP elution peaks in different water samples. 1-Ganga river at Dhashaswamedh ghat, 2-Varuna river at Chowka ghat, 3-Varuna river at Dhobi ghat, 4-Distilled water, 5-tap water, 6-tap water + standard, 7-4-NP treated water with fish (ohr), 8-4-NP treated water with fish (after 10hr). In Y axis scales are not the same.
Figure 4. Accumulation of 4-NP at low (64 μg/L) and high (160 μg/L) concentrations in different tissues (ng/mg tissue weight) of Heteropneustes fossilis during resting -preparatory phase. Data were expressed as mean ± SEM (n=5) and analyzed by two way ANOVA, followed by Newman-Keuls' test (p<0.05). * No significant difference between the controls at different durations. Groups with the same letter or symbol are not significantly different.
Figure 5. Accumulation of 4-NP at low (64 μg/L) and high (160 μg/L) concentrations in different tissues (ng/mg tissue weight) of Heteropneustes fossilis during preparatory- prespawning phase. Data were expressed as mean ± SEM (n=5) and analyzed by two way ANOVA, followed by Newman-Keuls' test (p<0.05). * No significant difference between the controls at different durations. Groups with the same letter or symbol are not significantly different.
Figure 6. Representative HPLC chromatograms showing 4-NP elution peaks in liver (preparatory phase, 60-day control) and different experimental tissue samples at 23.65 min. The unidentified peak at 27.23 ± 0.2 min is very prominent.
