Nutritional milieu of isolated stromal vascular cells determines their proliferative, adipogenic, and lipogenic capacity in vitro

Figures & data

Table 1. Fatty acid profile (g/100 g adipose tissue) of subcutaneous adipose tissue for donor steers that grazed legume pastures with (LC) or without (L) corn grain supplementation (0.75% of body weight/d)

Fatty acid	L	LC	SEM	P value
n	4	4
C14:0	2.51	3.67	0.1	0.01
C14:1c9	1.02	1.36	0.07	0.01
C16:0	20.40	24.47	0.85	0.01
C16:1c9	2.99	3.69	0.17	0.01
C18:0	9.99	10.41	0.53	0.58
C18:1 cis-9	27.54	31.7	1.34	0.04
C18:1 trans-11	1.68	1.21	0.13	0.02
C18:1 cis-11	0.76	0.90	0.05	0.05
C18:2 n-6	0.09	0.09	0.01	0.81
C18:2 cis-9 trans-11	0.60	0.58	0.04	0.75
C20:4 n-6	0.01	0.004	0.002	0.36
C20:5 n-3	0.001	0.002	0.001	0.64
C22:5 n-3	0.02	0.04	0.01	0.3
C22:6 n-3	0.008	0.008	0.001	0.8
SFA	32.90	38.55	1.38	0.01
MUFA	33.99	38.86	1.6	0.04
Total fatty acids	74.34	84.99	3.27	0.02

Table 2. Fatty acid profile (μg/well) of intermuscular adipocyte cultures at 6 d from steers that grazed legume pastures with (LC) or without (L) corn gain supplementation (0.75% of body weight/d). Cultures from each treatment group (L or LC) received 0 or 100 μM addition of linoleic (C18:2)

TRT	L	L	LC	LC		P value
C18:2	0	100	0	100		TRT	C18:2	TRTxC18:2
C10:0	3.26	2.76	2.92	3.43	0.43	0.70	0.99	0.25
C12:0	2.9	2.41	2.07	3.04	0.43	0.81	0.57	0.1
C14:0	0.91	0.82	0.64	1.03	0.38	0.93	0.7	0.54
C16:0	10.56	11.10	10.20	9.53	0.34	0.01	0.86	0.08
C16:1	0.54	0.55	0.39	0.25	0.06	0.01	0.35	0.28
C18:0	13.00	12.62	12.01	10.67	0.34	0.01	0.01	0.17
C18:1c9	9.62	7.79	5.46	3.23	0.85	0.01	0.02	0.81
C18:2	2.23^c	40.52^a	1.37^c	19.51^b	2.61	0.01	0.01	0.01
C20:4	5.62	2.89	4.97	2.48	0.16	0.01	0.01	0.47
C20:5	1.12	0.03	0.25	0.00	0.4	0.28	0.11	0.31
C22:5	1.46	1.24	1.28	1.15	0.05	0.02	0.01	0.39
C22:6	2.22	1.64	1.90	1.36	0.14	0.05	0.01	0.89
Total FA	112.10	149.97	98.95	118.57	7.44	0.01	0.01	0.23

^abMeans in the same row with uncommon superscripts differ (P < 0.05).

Figure 1. Cell proliferation of intermuscular preadipocytes at 24, 48, and 74 h in culture from steers that grazed legume pastures with (LC) or without (L) corn grain supplementation (0.75% of body weight/d). *Within a time point, LC group differs from L group (P < 0.05). **Time means differ from 24 h (P < 0.05).

Figure 2. Cell sizing [(A) diameter, μM; (B) volume, pL] of intermuscular adipocyte cultures from steers that grazed legume pastures with (LC) or without (L) corn grain supplementation (0.75% of body weight/d) at various days in culture. *Within a time point, LC group differs from L group (P < 0.05). Each time point with a different alphabet differs significantly (P < 0.05).

Figure 3. Cell size distribution of intermuscular adipocyte cultures at different days in culture from steers that grazed legumes with (LC) or without (L) corn grain supplementation (0.75% of body weight/d). Differentiation cocktail was added on d0. (A) L group, (B) LC group, and (C) Distribution.

Figure 4. Relative mRNA expression of bovine intermuscular adipocytes at various days (d −2, 2, 4, 6, and 12) in culture. Bovine preadipocytes were isolated from steers that grazed legume pastures with (LC) or without (L) corn grain supplementation (0.75% of body weight/d) and differentiation cocktail added on d0 to stimulate differentiation. *Within a time point, LC group differs from L group (P < 0.05).

Figure 5. Lipogenesis in bovine intermuscular adipocyte cultures from steers that grazed legume pastures with (LC) or without (L) corn grain supplementation (0.75% of body weight/d). (A) Estimation of molar percent excess (MPE) of acetate after [1-¹³C]2 addition to the media. (B) Tracer-to-tracee (TTR) ratio of C16:0. (C) Tracer-to-tracee (TTR) ratio of C16:0. (D) Tracer-to-tracee (TTR) ratio of C18:0. (E) Tracer-to-tracee (TTR) ratio of C18:0. (F) Fractional synthetic rate of ¹³C-16:0 synthesized from 1,2-¹³C-acetate over a period of 48 h. (G) Fractional synthetic rate of ¹³C-18:0 synthesized from 1,2-¹³C-acetate over a period of 48 h.

Figure 6. Glycerol 3-phosphate dehydrogenase activity of intermuscular adipocyte cultures at 6 and 12 d of culture from steers that grazed legume pastures with (LC) or without (L) corn grain supplementation (0.75% of body weight/d). *Within a time point, LC group differs from L group (P < 0.05). **Time means differ from 24 h (P < 0.05).

Figure 7. Photomicrographs of the intermuscular preadipocytes and adipocytes after differentiation from steers that grazed legume pasture with (LC) or without (L) corn grain supplementation (0.75% of body weight/d).