Figure 1. LC3-II formation is blocked only temporarily after ATG5 knockdown. Western blot analysis is shown of HeLa cells treated for 6 h ± 20 µM chloroquine to block basal autophagic flux of PLK0.1-shRNA nonsilencing (control shRNA) compared with shRNA targeting ATG5 cultured postinfection for up to 32 d. Although the knockdown worked well at 5 d postinfection through 26 d, basal autophagy was only blocked effectively in the samples tested at 11 and 15 d after infection.
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
To request a reprint or corporate permissions for this article, please click on the relevant link below:
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
Obtain permissions instantly via Rightslink by clicking on the button below:
If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.
