Cross-system excision of chaperone-mediated proteolysis in chaperone-assisted recombinant protein production

Figures & data

Figure 1 Model of DnaK and DnaJ differential action in bacterial and eukaryotic systems. By rehosting the chaperones to a system lacking orthologs of the bacterial proteases Lon and ClpP, proteolysis can be avoided while keeping the conserved foldase activity of the DnaK/J pair.