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Research Paper

Bacterial consortia constructed for the decomposition of Agave biomass

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Pages 165-172 | Received 17 Feb 2014, Accepted 04 Mar 2014, Published online: 05 Mar 2014

Figures & data

Table 1. Bacterial strains and their lignocellulose degrading characteristics for consortia development

Figure 1. Reducing sugar equivalents (µM) released by eight individual isolates during aerobic incubation over 7 d with 1% (w/v) microcrystalline cellulose and 1% (w/v) beechwood xylan.

Figure 1. Reducing sugar equivalents (µM) released by eight individual isolates during aerobic incubation over 7 d with 1% (w/v) microcrystalline cellulose and 1% (w/v) beechwood xylan.

Figure 2. Reducing sugar equivalents (µM) released from (A) first 5 groups of bacterial co-cultures and (B) an additional 5 bacterial co-cultures, during aerobic incubation over 7 d with imitation media: 0.5% (w/v) microcrystalline cellulose and 0.5% (w/v) beechwood xylan.

Figure 2. Reducing sugar equivalents (µM) released from (A) first 5 groups of bacterial co-cultures and (B) an additional 5 bacterial co-cultures, during aerobic incubation over 7 d with imitation media: 0.5% (w/v) microcrystalline cellulose and 0.5% (w/v) beechwood xylan.

Figure 3. (A) Reducing sugar equivalents (µM) released by 2 tetra-culture and 4 tri-cultures of bacteria during aerobic incubation over 14 d with imitation media: 0.5% (w/v) microcrystalline cellulose and 0.5% (w/v) beechwood xylan. (B) Bacterial consortia mixed cell densities in log(CFU/ml) for bacterial consortia grown in imitation media for 14 d. Consortia: C + A1 + G + A4 (♦), C + A1 + G + 5 (□), G + A1 + A4 (Δ), C + A1 + G (x), G + A1 + 5 (+), G + A4 + 5 (○).

Figure 3. (A) Reducing sugar equivalents (µM) released by 2 tetra-culture and 4 tri-cultures of bacteria during aerobic incubation over 14 d with imitation media: 0.5% (w/v) microcrystalline cellulose and 0.5% (w/v) beechwood xylan. (B) Bacterial consortia mixed cell densities in log(CFU/ml) for bacterial consortia grown in imitation media for 14 d. Consortia: C + A1 + G + A4 (♦), C + A1 + G + 5 (□), G + A1 + A4 (Δ), C + A1 + G (x), G + A1 + 5 (+), G + A4 + 5 (○).

Figure 4. (A) Bacterial consortia mixed cell growth in log(CFU/ml) after 14 d growth with ground Agave. Consortia: C + A1(♦), C + A4 (□), C + 5 (Δ), G + A1 (x), G + A4 (*), G + 5 (○), G + A1 + 5 (+), and G + A1 + A4 (‒). (B) Agave decomposition expressed as the dry weight percentage (%) compared with the control.

Figure 4. (A) Bacterial consortia mixed cell growth in log(CFU/ml) after 14 d growth with ground Agave. Consortia: C + A1(♦), C + A4 (□), C + 5 (Δ), G + A1 (x), G + A4 (*), G + 5 (○), G + A1 + 5 (+), and G + A1 + A4 (‒). (B) Agave decomposition expressed as the dry weight percentage (%) compared with the control.

Figure 5. Scanning electron images of Agave surface after bacterial consortia: (B) C + A1, (C) C + A4, (D) C + 5, (E) G + A1, (F) G + A4, (G) G + 5, (H) G + A1 + 5, and (I) G + A1 + A4, treatment for 14 d compared with (A) control (no bacteria).

Figure 5. Scanning electron images of Agave surface after bacterial consortia: (B) C + A1, (C) C + A4, (D) C + 5, (E) G + A1, (F) G + A4, (G) G + 5, (H) G + A1 + 5, and (I) G + A1 + A4, treatment for 14 d compared with (A) control (no bacteria).

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