Figures & data
Table 1. Composition of LGI-P, Y&P-NaCl, high P, and low P media
Figure 1.G. diazotrophicus Pal5 WT, gumD mutant, and lsdA mutant strains grown on high or low P solid medium. Plus (+++) indicates the strongest mucous trait, plus (+) is moderate, and minus (-) is the weakest. Cells were grown for 1 wk at 30 °C.
Figure 2.G. diazotrophicus Pal 5 WT grown on high P, low P, and low P medium + NaCl containing the same concentration of Na+ as that of the high P medium. Cells were grown for 1 wk at 30 °C.
Figure 3. HPLC analysis of mucous materials. Authentic levan and mucous material collected from colonies of WT, gumD, and lsdA cells grown for 1 wk at 30 °C on the indicated solid media were hydrolyzed and analyzed. The vertical axis of the chromatograph shows the relative peak level. The retention time (min) is shown on the horizontal axis. Arrows show the levan peak (15.3 min).
Table 4. Concentration of phosphate in sugarcane juice
Figure 4. Growth on high P or low P medium of bacteria isolated from sugarcane juice. Plus (+++) indicates the strongest mucous trait, plus (+) is moderate, and minus (-) is the weakest. Cells were grown for 1 wk at 30 °C. Bacteria were tentatively identified based on the rDNA sequences.
Figure 5. Growth of G. diazotrophicus Pal5 WT and lsdA disruptant on solid media containing hydrogen peroxide (H2O2). Three spotted cells were diluted 10-fold from OD600 of 1.0 to 0.01 from right to left.
