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COMMENTARY

Multiwalled carbon nanotubes enhance human bone marrow mesenchymal stem cells’ spreading but delay their proliferation in the direction of differentiation acceleration

Pages 558-562 | Received 30 Sep 2013, Accepted 24 Jul 2014, Published online: 26 Jan 2015

Figures & data

Figure 1. Fluorescent staining of the f-actin cytoskeleton (green) and DNA (blue), showing cell spreading. (A) 6 h on MWCNTs. (B) 3rd day on MWCNTs. (C) 6 h on TCP. (B) 3rd day on TCP.

Figure 1. Fluorescent staining of the f-actin cytoskeleton (green) and DNA (blue), showing cell spreading. (A) 6 h on MWCNTs. (B) 3rd day on MWCNTs. (C) 6 h on TCP. (B) 3rd day on TCP.

Figure 2. SEM images showing cytoplasmic processes and filopodia. (A–C) 3rd day on MWCNTs. (D) 3rd day on TCP.

Figure 2. SEM images showing cytoplasmic processes and filopodia. (A–C) 3rd day on MWCNTs. (D) 3rd day on TCP.

Figure 3. Quantitative real time RT-PCR gene expression analysis of vinculin gene.

Figure 3. Quantitative real time RT-PCR gene expression analysis of vinculin gene.

Figure 4. Lactate dehydrogenase (LDH) activity measured in cell supernatant after 24 h of culture on MWCTs and on TCP.

Figure 4. Lactate dehydrogenase (LDH) activity measured in cell supernatant after 24 h of culture on MWCTs and on TCP.

Figure 5. Total-protein of the hMSCs cells after 1, 3 and 7 d of culture on 2 different concentrations of MWCNTs. CNTs_50: 50 μg/mL, CNTs_200: 200 μg/mL and on TCP. Images of cultured cells on the substrates are also shown.

Figure 5. Total-protein of the hMSCs cells after 1, 3 and 7 d of culture on 2 different concentrations of MWCNTs. CNTs_50: 50 μg/mL, CNTs_200: 200 μg/mL and on TCP. Images of cultured cells on the substrates are also shown.

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