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Commentary & View

Eph-ephrin bidirectional signaling comes into the context of lymphocyte transendothelial migration

, &
Pages 363-367 | Received 18 Dec 2009, Accepted 22 Feb 2010, Published online: 01 Jul 2010

Figures & data

Figure 1 EphA2 is differentially expressed in the CD31+ vascular vessels of human lymph nodes. 8 µm thick cryosections from human lymph node biopsies of either control subjects (reactive lymph node) (left) or CLL patients (right) were immunostained with a primary polyclonal antibody for human EphA2 followed by AlexaFluor-546 conjugated specie-specific secondary antibody (shown in red) and an AlexaFluor-488 conjugated anti human CD31 (shown in green). representative experiments are shown. Fluorescence images were acquired with a confocal microscope (Leica TCS-SP2 AOBS; objective: 20x multi-immersion, 1.20 NA).

Figure 1 EphA2 is differentially expressed in the CD31+ vascular vessels of human lymph nodes. 8 µm thick cryosections from human lymph node biopsies of either control subjects (reactive lymph node) (left) or CLL patients (right) were immunostained with a primary polyclonal antibody for human EphA2 followed by AlexaFluor-546 conjugated specie-specific secondary antibody (shown in red) and an AlexaFluor-488 conjugated anti human CD31 (shown in green). representative experiments are shown. Fluorescence images were acquired with a confocal microscope (Leica TCS-SP2 AOBS; objective: 20x multi-immersion, 1.20 NA).

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