Figures & data
Figure 1. Functional assays of Ki-CA patient tumor derived cells’ response to molecular targeted small-molecule kinase inhibitors. Two thousand cells in medium without EGF were added to 96-well plates containing each small-molecule inhibitor at four serial dilutions spanning a concentration range that includes the predicted IC50, incubated at 37°C for three days and assayed by MTS (Promega). The viability data were adjusted for wells with no cells/inhibitor, and normalized to untreated wells of the cultured cells (OD value for wells with cells without drug treatment = 100% cell viability) and to a database of cell lines and tumor samplesCitation4 to yield responses and the IC50 in nM for each agent. IC50 values less than 20% of the global median (shown as 100%) are considered responses and are well within clinically achievable concentrations.
Figure 2. Dose responses of Ki-CA patient derived cell line to selected drugs. Cells were added to 96-well plates containing each small-molecule inhibitor at 11 serial dilutions spanning a concentration range that includes the predicted IC50 and evaluated as in . Values shown are mean ± SD for triplicate wells. Best fit curves were generated using GraphPad Prism software.
Figure 3. Primary Ki-CA patient tumor immunohistochemistry. Tumor exhibited both Src positivity (A) and VHL positivity (B). Power: 20 × ; Scale bar: 20 μM
