Figures & data
Figure 1 Identification of genes repressed by GSK-3. Microarray analysis was used to identify immediate early genes that were induced by 30 min of PDGF stimulation of quiescent T98G cells.Citation12 Small-molecule inhibitors were used to identify genes that were dependent on PI 3-kinase and MEK/ERK for their induction. The genes showing PI 3-kinase dependence were subsequently tested for their ability to be induced by direct inhibition of GSK-3 (using the small-molecule inhibitor SB-216763) in the absence of growth factor stimulation.Citation18
Figure 2 GSK-3 transcriptional network in quiescent cells. Grey and black arrows indicate positive ChIP binding by the indicated transcription factor. Black arrows indicate that siRNA against the transcription factor blocked induction of the gene upon direct inhibition of GSK-3 using the small-molecule inhibitor SB-216763. The blunt-ended line between AP-1 and NR4A1 indicates an inhibition of induction in the presence of AP-1. RND3 and CCL8 are excluded, as no functional connections could be established with AP-1, NFκB or CREB.
Table 1 Functional categories of the GSK-3-repressed genes