Figures & data
Figure 1 Replication origins are licensed after the loading of MCM2–7 helicase complexes. This process is conserved in all eukaryotes and requires the action of Cdt1 and Cdc6, which are recruited in G1-phase together with Orc1–6 (pre-RC). In S-phase, active CDKs and DDKs allow MCMs activation thanks to the recruitment of Cdc45 and other additional factors to the pre-RC. In yeast, Sld2 and Sld3 are the main CDK targets required for this step; they both interact with Dpb11 leading to Cdc45 recruitment and origin firing. In higher eukaryotes different Sld2/3 like-proteins (pentagons) are involved in this step. In particular, Gemc1 is phosphorylated by CDK and interacts with TopBP1 for the recruitment of Cdc45.
Figure 2 Possible roles of GEMC1 in DNA damage condition (see text).
