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Article Addendum

mDia3-EB1-APC

A connection between kinetochores and microtubule plus ends

&
Pages 480-482 | Received 28 Mar 2011, Accepted 28 Mar 2011, Published online: 01 Jul 2011

Figures & data

Figure 1 A Model for kinetochores tracking spindle microtubule plus-ends. During metaphase chromosome oscillation, the leading and trailing kinetochores in a sister kinetochore pair have two distinct stably attached microtubule populations: the trailing kinetochore is attached to growing microtubules, whereas the leading kinetochore retains its attachment to shrinking microtubules. The Ndc80 complex is a central component in stabilizing end-on kinetochore microtubule attachment. Microtubule plus-end tracking proteins, EB1 and APC, track plus ends of growing microtubules. The interaction between EB1-APC and formin mDia3, a novel kinetochore protein, may mediate continuous Ndc80 tip tracking with growing microtubules, either directly or through other unknown linker proteins. The Ska complex is able to mediate a cargo association with depolymerizing microtubule plus ends in vitro.Citation27 Whether the Ska complex is able to interact with Ndc80 and increase the stability of connection between Ndc80 and shrinking microtubule ends is not clear.

Figure 1 A Model for kinetochores tracking spindle microtubule plus-ends. During metaphase chromosome oscillation, the leading and trailing kinetochores in a sister kinetochore pair have two distinct stably attached microtubule populations: the trailing kinetochore is attached to growing microtubules, whereas the leading kinetochore retains its attachment to shrinking microtubules. The Ndc80 complex is a central component in stabilizing end-on kinetochore microtubule attachment. Microtubule plus-end tracking proteins, EB1 and APC, track plus ends of growing microtubules. The interaction between EB1-APC and formin mDia3, a novel kinetochore protein, may mediate continuous Ndc80 tip tracking with growing microtubules, either directly or through other unknown linker proteins. The Ska complex is able to mediate a cargo association with depolymerizing microtubule plus ends in vitro.Citation27 Whether the Ska complex is able to interact with Ndc80 and increase the stability of connection between Ndc80 and shrinking microtubule ends is not clear.