Figures & data
Figure 1 Effects of NO on externalization of endogenous tTG in HAECs. (A) Untreated cells and cells pretreated with NO donor GSNO (200 µm, 1 h) or NOS inhibitor L-NAME (100 µm, 18 h), were metabolically labeled with 35S-translabel for 0–8 h. at the end of labeling, cells were surface-biotinylated with Sulfo-NHS-LC-biotin and cell surface protein fractions were isolated on neutravidinagarose.Citation5,Citation7 the de novo synthesized tTG was immunoprecipitated from total cell extracts (left parts) and cell surface protein fractions (right parts). the resulting tTG immune complexes were resolved by SDS-PAGE and visualized by fluorography. Large and small arrowheads mark the positions of tTG (∼80 kDa) and its major proteolytic fragment (∼68 kDa). (B) the relative amounts of de novo synthesized cell surface tTG were quantified by scintillation counting and compared to those of total tTG. Shown in (A) is a representative of four independent experiments. Bars in (B) depict means ± Sem. *p < 0.05.
Figure 2 A scheme depicting the effects of exogenous and endogenously produced NO on nonclassical secretion of cytoplasmic tTG.
