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Article Addendum

Redox regulation of phototactic migration in the green alga Chlamydomonas reinhardtii and its possible application

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Pages 196-198 | Published online: 01 Mar 2012

Figures & data

Figure 1. Summary of the effects of ROS or ROS scavengers on the phototactic sign. Polar histograms depict the percentage of cells moving in a particular direction relative to light illuminated from the right, with or without treatment with redox reagents (12 bins of 30°, n = 50 cells per condition). Wild type cells exhibited positive whereas agg1 mutant cells exhibited negative phototaxis without treatment with any reagents. Note that wild type cells exhibited negative phototaxis after treatment with ROS scavengers (blue), whereas agg1 cells showed positive phototaxis after treatment with ROS (red) (histograms in yellow boxes). Modified from Figure 2 in Wakabayashi et al.Citation5

Figure 1. Summary of the effects of ROS or ROS scavengers on the phototactic sign. Polar histograms depict the percentage of cells moving in a particular direction relative to light illuminated from the right, with or without treatment with redox reagents (12 bins of 30°, n = 50 cells per condition). Wild type cells exhibited positive whereas agg1 mutant cells exhibited negative phototaxis without treatment with any reagents. Note that wild type cells exhibited negative phototaxis after treatment with ROS scavengers (blue), whereas agg1 cells showed positive phototaxis after treatment with ROS (red) (histograms in yellow boxes). Modified from Figure 2 in Wakabayashi et al.Citation5

Figure 2. Concentration of Chlamydomonas cells based on the redox regulation of phototaxis. About 1-liter culture of wild type cells were treated with 75 mM dimethylthiourea (DMTU) and illuminated with a green LED from the top.

Figure 2. Concentration of Chlamydomonas cells based on the redox regulation of phototaxis. About 1-liter culture of wild type cells were treated with 75 mM dimethylthiourea (DMTU) and illuminated with a green LED from the top.