Figures & data
Figure 1. Regulatory dynamics during cytokinesis in S. pombe. (A) During interphase, Mbx1p, a component of the pombe cell cycle box binding factor (PBF), is bound and dephosphorylated by Clp1p. In this form Mbx1p aids in repression of the transcription of a set of genes (including mid1, sid2, and cdc15) with roles in cytokinesis. (B) During normal cytokinesis—or upon extension of the cytokinetic (or “C”) phase of the cell cycle upon perturbation of the cell division machinery—Plo1p phosphorylates Mbx1p, relieving its inhibitory effect on the transcription of cytokinesis genes such as sid2. Sid2p phosphorylates Clp1p, creating a binding site for the 14-3-3 protein, Rad24p. Rad24p binding to Clp1p promotes the cytoplasmic retention of Clp1p, preventing it from de-phosphorylating Mbx1p.
