Figures & data
Figure 1. (A) Growth of wild-type (wt) and cdc42L160S cells spotted at an A600 of 1.0 and 1/4 dilutions in rich medium without or with 1.2M sorbitol. Plates were incubated at different temperatures for 3 d. (B) Cell wall composition of wild-type (wt) and cdc42L160S strains. Cells were incubated at 28°C or 36°C and 14C-glucose was added 6 h before harvesting the cells. Values are the mean of three independent experiments with duplicate samples. Error bars represent standard deviations for the total carbohydrate values. (C) Fluorescence images of GFP-Bgs1 and GFP-Bgs4 in wild-type (wt) and cdc42L160S cells grown at 32°C. (D) Recovery of GFP-Bgs4 in wild-type and cdc42L160S cells. Photo-bleaching of a small region at the tip was accomplished using a Delta Vision system by applying 488-nm laser to cell tips. Images were acquired prior to photobleaching, immediately after and subsequently at regular 10 sec intervals. Left panel shows the average recovery of GFP-Bgs4 (n = 10) in this experiment. (E) Photo-bleaching of the entire cell tip was accomplished using a Leica confocal system by applying 488-nm laser to a region of 40x10 pixels. Images were acquired prior to photobleaching, immediately after and subsequently at regular 1 min intervals.
Figure 2. Cdc42 and trafficking pathways in fission yeast. Proteins can be secreted or targeted to the cell surface in secretory vesicles (SV) that are directed via actin cables and are tethered by the exocyst protein complex to specific sites of the plasma membrane. Molecules taken up from the plasma membrane are delivered to early endosomes by an endocytic process that might lead to a route of recycling or to complete degradation in the vacuole. Cdc42 regulates the actin-directed vesicle delivery to the plasma membrane, the exocyst function, and the endosome to vacuole fusion.
