Figures & data
Figure 1 EGF stimulation induces coalescence of GM1-containing rafts. Serum-starved HER14 cells (NIH 3T3 cells stably expressing human EGFR), were labeled with 1 µg/ml cholera toxin B-subunit conjugated to Alexa Fluor 488 (CTB-A488) and/or Alexa Fluor 594 (CTB-A594) on ice. After 1 hour, the cells were recovered to 37°C and stimulated with 20 ng/ml EGF for 1 or 10 minutes. The cells were fixed with 4% formaldehyde and the coverslips were embedded in mowiol. The lifetimes of Alexa 488 were determined by FLIM analysis as described previously.Citation9 Histograms show the average lifetime values of four cells per condition (*p < 0.05).
