Figures & data
Figure 1. (A) MICAL-L1 localizes to tubular recycling endosomes. HeLa cells grown on coverslips were fixed, permeabilized and incubated with anti-MICAL-L1 antibody. Cells were then incubated with Alexa-Fluor-conjugated anti-mouse antibody and DAPI. (B) Model depicting MICAL-L1 interaction partners and functional interplay with various trafficking regulators. Through its CH and LIM domains, MICAL-L1 binds to CRMP2 to modulate trafficking via kinesin and dynein motor proteins. MICAL-L1 associates with EHD1 through its first NPF motif that is followed by a cluster of acidic residues, thus controlling the localization of EHD1 to tubular membranes. MICAL-L1, a Rab35 effector, binds to Rab35, Rab8, Rab10, and Arf6 through its CC region. Rab35 regulates MICAL-L1 localization to tubular membranes and also controls Arf6 activation through ACAP2. Arf6 controls MICAL-L1 localization to tubular endosomes and in turn, MICAL-L1 controls Rab8 localization. Dotted lines indicate a direct association between proteins. Solid lines indicate a functional interplay between the proteins, where arrowheads point toward the protein being regulated. Diagram is not drawn to scale.
