Figures & data
Figure 1. ARF6 regulates cellular ODN uptake. RAW264.7, RAW-ARF1T31N, RAW-ARF6T27N or RAW-ARF6Q71L cells were incubated with 0.5 μM GpC ODN-FITC or CpG ODN-FITC for 30 min. Cell fluorescence intensity of GpC ODN-FITC or CpG ODN-FITC uptake was measured by flow cytometry. *p < 0.01 for RAW-ARF6T27N or RAW-ARF6Q71Lvs. RAW264.7.
Figure 2. CpG ODN pre-treated cells increase cellular ODN uptake. RAW264.7 cells were pre-treated with either CpG ODN or GpC ODN for 30 min, followed by 0.5 μM GpC ODN-FITC or CpG ODN-FITC for 10 min. (A) The percentages of FITC-positive cells in total cells counted and (B) Cell fluorescence intensity of GpC ODN-FITC or CpG ODN-FITC uptake was measured by flow cytometry. Data represent the mean ± SD of 3 experiments. *p < 0.01 for CpG ODN pre-treated cells vs. GpC ODN pre-treated cells.
Figure 3. CpG ODN induces recruitment of ARF6 to plasma membrane. RAW-ARF6 cells were treated with CpG ODN for the indicated times and then the distribution of ARF6 on plasma membrane was assessed by immunoblot with anti-HA and anti-EGFR (membrane protein marker).
