Figures & data
Table 1. Failure of in vitro expansion of cybrid embryonic cells
Figure 1. Defective in vitro expansion of cybrids cells. The concentration of control haploid [l]xl embryonic cells (A-C) in a given area of a culture dish visibly increased over time, while that of [l]xt cybrid cells (D-F) did not. Pictures in (B, E) and (C, F) were taken 5 and 13 d, respectively, after those in (A, D). Scale bar: 0.1 mm.
![Figure 1. Defective in vitro expansion of cybrids cells. The concentration of control haploid [l]xl embryonic cells (A-C) in a given area of a culture dish visibly increased over time, while that of [l]xt cybrid cells (D-F) did not. Pictures in (B, E) and (C, F) were taken 5 and 13 d, respectively, after those in (A, D). Scale bar: 0.1 mm.](/cms/asset/d0d4ea06-0db7-4d85-8dbc-b3f4de0372bc/kcib_a_10920334_f0001.gif)
Figure 2. Improved cybrid embryo development by nuclear species maternal poly(A)+ RNA injection. The most developed [l]xt cybrid embryo that was obtained following injection at the 1-cell stage with (A) dH20, (B) X. laevis oocyte poly(A)+ RNA, or (C) X. tropicalis oocyte poly(A)+ RNA from one experiment are shown as an example. All three embryos had a rudimentary sucker (arrowhead), yet noticeable improvements in the development of the animal shown in (C) include a better blastopore closure (green dotted line), axis formation and elongation (red dotted line), muscular response, and an increased head size (yellow dotted line). Embryos are shown (anterior to the right; dorsal up) at 48 h post-fertilization, about 24 h before they were finally scored for inclusion in . Scale bar: 1 mm.
![Figure 2. Improved cybrid embryo development by nuclear species maternal poly(A)+ RNA injection. The most developed [l]xt cybrid embryo that was obtained following injection at the 1-cell stage with (A) dH20, (B) X. laevis oocyte poly(A)+ RNA, or (C) X. tropicalis oocyte poly(A)+ RNA from one experiment are shown as an example. All three embryos had a rudimentary sucker (arrowhead), yet noticeable improvements in the development of the animal shown in (C) include a better blastopore closure (green dotted line), axis formation and elongation (red dotted line), muscular response, and an increased head size (yellow dotted line). Embryos are shown (anterior to the right; dorsal up) at 48 h post-fertilization, about 24 h before they were finally scored for inclusion in Table 2. Scale bar: 1 mm.](/cms/asset/99105535-4841-4b75-86b2-ea2ebd0fa4a3/kcib_a_10920334_f0002.gif)
