352
Views
4
CrossRef citations to date
0
Altmetric
Article Addendum

Intracellular cryptococci suppress Fc-mediated cyclin D1 elevation

&
Pages 390-391 | Received 13 Apr 2010, Accepted 13 Apr 2010, Published online: 01 Jul 2010

Figures & data

Figure 1 Western blots of Cyclin D1 in bone marrow macrophage cell extract after Fc-mediated phagocytosis of live Cn or polystyrene beads. Bone marrow macrophage cells were challenged with mAb 18B7-opsonized live Cn or polystyrene beads at an E:T ratio of 1:1. In the negative control group, mAb 18B7 without the presence of particles was added. Macrophage cell contents were extracted after 0, 0.5, 2, 4 and 6 h post challenge. Cyclin D1 expression levels in the cell extracts after phagocytosis assay were probed by western blots. The same loading amount for each sample was confirmed by β-tubulin western blots. The experiment has been independently repeated three times and produced similar results.

Figure 1 Western blots of Cyclin D1 in bone marrow macrophage cell extract after Fc-mediated phagocytosis of live Cn or polystyrene beads. Bone marrow macrophage cells were challenged with mAb 18B7-opsonized live Cn or polystyrene beads at an E:T ratio of 1:1. In the negative control group, mAb 18B7 without the presence of particles was added. Macrophage cell contents were extracted after 0, 0.5, 2, 4 and 6 h post challenge. Cyclin D1 expression levels in the cell extracts after phagocytosis assay were probed by western blots. The same loading amount for each sample was confirmed by β-tubulin western blots. The experiment has been independently repeated three times and produced similar results.