Figures & data
Figure 1 Cortactin is co-phosphorylated on S405 and Y421. OSC19 oral squamous carcinoma cells serum starved for 24 h were stimulated with 100 ng/ml EGF for 20 min as indicated. Cell lysates were immunoprecipitated with anti-cortactin pS405 antibodies and analyzed by western blotting with anti-cortactin pY421 and pan-cortactin antibodies. Total cell lysates were evaluated for the presence of pS405 cortactin, pY421 cortactin, total cortactin and β-actin.
Figure 2 Mechanistic and cellular roles of cortactin tyrosine and serine phosphorylation in tumor cell motility. Tyrosine (purple) and serine (green) phosphorylation events are shown depicting direct and indirect pathways that ultimately result in N-WASp activation. The effects of cortactin serine and tyrosine phosphorylation result in common net outcomes in invadopodia function (ECM degradation), but differ in impact on lamellipodia dynamics as shown. Cortactin phosphorylated on serine and tyrosine residues is enriched in human tumors, where it likely enhances invasive and metastatic capacity.
