Endosomal and secretory markers of the Legionella-containing vacuole

Figures & data

Figure 1 Communication of LCVs with endosomal and secretory trafficking pathways. LCVs are decorated with endosomal and secretory markers, which might be acquired by (i) direct fusion with cell organelles, (ii) interaction with transport vesicles shuttling between compartments, or (iii) recruitment from the cytoplasm. L. pneumophila produces two distinct classes of Icm/Dot-translocated effector proteins, which interact with PtdIns(4)P (SidC, SidM)5,18,35 or PtdIns(3)P (LpnE, LidA),15,35 respectively.

Figure 2 GST-Rab8 interacts with the Icm/Dot substrate LidA in L. pneumophila lysates. GST fusions proteins of Rab8 wild-type (wt) or constitutive active (ca; Rab8_Q74L36) were used to precipitate binding partners in L. pneumophila lysates. Protein bands eluting from washed Rab8-coated beads but not from control beads were identified by LC-MS/MS as the Icm/Dot substrate LidA, the Rab1 GEF SidM and a putative tellurite resistance protein (TRP).

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