High quality methylome-wide investigations through next-generation sequencing of DNA from a single archived dry blood spot

Figures & data

Figure 1. Sequence quality of methylome-wide data. Data quality outcome variables (y-axis) are given for the samples from whole blood (averages from all samples are shown) and from all blood spots with 2 µg starting material (averages from all samples are shown) as well as for all conditions for the two samples (sample A and B) where different amounts of starting material were used (x-axis).

Figure 2. Overlap with biological features. The percentage of covered CpG sites overlapping with biological features are given for the whole blood (averages from all samples are shown) and from all blood spots with 2 µg starting material (averages from all samples are shown) as well as for all conditions for the two samples (sample A and B) where different amounts of starting material were used (x-axis).

Table 1. Study samples and amount of starting material used for each methylome-wide profiling

Individual	No. spots	Years stored	DNA extracted (µg)	2.0 µg	1.0 µg	0.5 µg	0.25 µg
1	2	18	2.0	x
2	3	18	3.9	x	x	x	x
3	2	19	2.6	x		x
4	3	16	2.7	x

Note. “No. spots” is the total number of spots from which DNA was extracted. Years stored indicates for how long the blood spots have been stored prior to DNA extraction. DNA extracted indicates the total amount of DNA extracted. The amount of starting material for each methylome investigation is indicated with an x.