Figures & data
Figure 1. (A and B) Detectable range (sensitivity) of Pyrosequencing for Imprinted Expression (PIE). PIE precisely reflected the theoretical ratio of the 2 alleles for both mouse (A) and human (B) IGF2. The trendline showed perfect linearity (R2 > 0.99) for both assays, and 1% changes were able to be detected. The graphs were generated from 3 independent experiments and the data shown as Mean ± SD. (C and D) Accuracy (specificity) of PIE. Pyrosequencing measured equal amounts of both alleles in genomic DNA from heterozygous mice (C), but only demonstrated expression from one allele using mRNA (D) when the intron-crossing primers were used. (E) PIE demonstrates LOI at IGF2 in prostate tissues associated with prostate cancer. Five normal prostates (NTA) with no evidence of inflammation or cancer and 5 prostate tumor tissues (T) were analyzed. Prostate tumor showed significantly LOI of IGF2 compared with NTA tissue. Data are shown as mean ± SD, * t-test p < 0.05.
