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Article Addendum

Reprofiled drug targets ancient protozoans

Drug discovery for parasitic diarrheal diseases

, &
Pages 66-71 | Published online: 08 Nov 2012

Figures & data

Figure 1. High throughput compound screening protocol for E. histolytica and G. lamblia trophozoites in 96-well microtiter plate.

Figure 1. High throughput compound screening protocol for E. histolytica and G. lamblia trophozoites in 96-well microtiter plate.

Figure 2. In vitro efficacy of different concentrations of aurnofin on the growth of C. parvum, as determined by real-time PCR. Bars represent standard errors of the means derived from three replicates.

Figure 2. In vitro efficacy of different concentrations of aurnofin on the growth of C. parvum, as determined by real-time PCR. Bars represent standard errors of the means derived from three replicates.

Figure 3. Clustered display of data from a portion of E. histolytica microarray, indicating downregulated (A) and upregulated transcripts (B) due to auranofin treatment. Microarray hybridizations (A and B) were performed using RNA from DMSO-treated E. histolytica and 1 µM auranofin-treated E. histolytica. Data from related genes in two separate hybridizations (Eh34 and Eh35) were analyzed by use of Acuity software.Citation22

Figure 3. Clustered display of data from a portion of E. histolytica microarray, indicating downregulated (A) and upregulated transcripts (B) due to auranofin treatment. Microarray hybridizations (A and B) were performed using RNA from DMSO-treated E. histolytica and 1 µM auranofin-treated E. histolytica. Data from related genes in two separate hybridizations (Eh34 and Eh35) were analyzed by use of Acuity software.Citation22

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