Paracoccidioidomycosis vaccine

Figures & data

Table 1. Protective immunizations with P10: adjuvants, peptide delivery and gene therapy

P10 adjuvant/delivery	Route immunization	Mouse strain	Reference
Complete Freund’s adjuvant	subcutaneous	Balb/c, A/Sn, C57Bl6	6
Multiple antigen peptide	subcutaneous	Balb/c	11
Flagellin genetically fused	intranasal	Balb/c	12
flanked by lysine residues	intranasal	Balb/c	12
P10 admixed with FliC flagellin	intranasal, subcutaneous	Balb/c	12, 13
Poly(lactic-glycolic acid)nanoparticles	intraperitoneal	Balb/c	15
pcDNA3-P10 (plasmid encoding P10)	intramuscular	Balb/c, B10.A	18
with pIL-12	intramuscular	Balb/c, B10.A	18
Dendritic cells primed with P10	subcutaneous, intravenous	Balb/c	16
Aluminum hydroxide	subcutaneous	Balb/c	13
Cationic lipid	subcutaneous	Balb/c	13

Figure 1. Histopathology of lungs from intratracheally infected BALB/c mice. Animals were infected with P. brasiliensis Pb18 for 30 d and treated with pcDNA3-P10 and pORF-mIL-12. (A and C) Untreated infected mice; (B and D) Infected mice immunized with plasmids pP10 and pIL-12 (A and B). Gomori staining showing yeast cells embedded in the lung infiltrate and clearing with plasmid treatment; (C and D) Masson’s trichrome staining showing blue collagen fibers, a granuloma and great number of yeast cells, and the effect of plasmid treatment. Original magnification, 100X.