Figures & data
Figure 1. Western blot analysis of HAC-VLPs in sucrose gradient fractions using an anti-HAC mAb (A). Monomeric HA (HAC1) was used as a positive control. HAC-VLPs recovered after sucrose gradient fractionation were analyzed by EM using negative staining (B) and immunogold labeling (C).
Figure 2. Serum HI antibody titers in mice immunized with HAC-VLPs and the percent responders per group. Data are shown as the average HI antibody titer per group plus SEM. The numbers on the top of each bar indicate the percent responders per group, meaning the percent of mice per group generating a HI titer of ≥1:40. (A): Post primary immunization (study day 21). (B): Post boost immunization (study day 42). Statistical analysis was performed to compare HI antibody titers in HAC-VLP immunized vs. HAC1 immunized groups by the Mann-Whitney testing using GraphPad Prism ver. 6.02. **, P < 0.01; ****, P < 0.0001; no asterisk, P > 0.05.
Figure 3. Total IgG (A) and IgG1/IgG2a (B) responses after immunization. Data are shown as the mean titer per group plus SEM. Statistical analysis was performed to compare IgG1 and IgG2a responses in HAC-VLP immunized vs. HAC1 immunized groups by the Mann-Whitney test using GraphPad Prism ver. 6.02. **, P < 0.01; no asterisk, P > 0.05.
