Figures & data
Figure 1 Manumycin alters nuclear morphology. GFP::Ce-lamin in live control and manumycin treated worms at days 4 (upper part) and 6 (lower part) of adulthood. C. elegans were treated with 125, 250 and 500 nM of manumycin and grown at 20°C. Nuclei in worms treated with manumycin show fewer convolutions compared to nuclei from untreated worms. Bar = 10 microns and applies to all parts.
Figure 2 Manumycin affects motility and is toxic to C. elegans. (A) Relative motility of control and manumycin-treated worms. Black bars indicate control; dark gray bars 125 nM manumycin and light gray bars 250 nM of manumycin. Y-axis shows relative average motility to control worms at day 1 of adulthood. (B) Survival plot of control and manumycin treated animals. The amount of manumycin is indicated next to each graph. The animal lifespan was shortened with increased doses of manumycin.
Figure 3 Nuclei retain their round morphology following polyprenyl synthetase downregulation by fdps-1(RNAi). (A) Animals were treated with either the empty vector L4440 (EV) or with fdps-1(RNAi) starting at the young adult stage (day 1). GFP::Ce-lamin was used to view changes in nuclear and nuclear envelope shapes. The fdps-1(RNAi)-treated nuclei showed some lamin aggregations at the nuclear periphery, but no lobulation. Bars = 10 microns.
Figure 4 Downregulation of polyprenyl synthetase by fdps-1(RNAi) blocks the redistribution of chromatin in nuclei of aging cells. Animals were treated with either L4440 vector (EV) or with fdps-1(RNAi) starting at the young adult stage (day 1). Ce-emerin::GFP and Hoechst DNA staining were used to view changes in nuclear envelope shapes and chromatin distribution. fdps-1(RNAi)-treated worms show diminished chromatin mislocalization at old age. In the merge parts green = lamin; blue = DNA. Bars = 10 microns.
Figure 5 Downregulation of polyprenyl synthetase by fdps-1(RNAi) has no significant effect on motility and lifespan. (A) Relative motility of control (dark gray) and fdps-1(RNAi) (light gray)-treated worms. (B) Survival plot of control- and fdps-1(RNAi)-treated animals. The black line indicates control and the light gray line fdps-1(RNAi).
