Figures & data
Figure 1. Transcriptional profiles analysis between NSCs cultured under 2-D and 3-D conditions. A. Unsupervised clustering analysis of the NSCs cultured in 3-D collagen scaffolds, 2-D PDL-coating dishes and 2-D collagen-coating dishes based on microarray expression data. B-D, scatter plot of microarray data comparing NSCs cultured in 3-D and 2-D culture conditions.
Figure 2. Morphology and pluripotency induction of MEFs by 3-D culture. A, The phase-contrast image of MEFs cultured on 2-D culture dish. B, The image of scanning electronic microscopy of MEFs cultured in 3-D collagen scaffolds. Diversity morphologies of cells in 3-D were observed with round (indicated by asterisk), shuttle shape-like (indicated by arrow) or spread-out appearances (indicated by triangle). C, qPCR analysis of ES marker genes in MEFs cultured under 3-D and 2-D conditions for 12 d. *p < 0.001.
