Direct Detection of Disease-Associated Prions in Brain and Lymphoid Tissue Using Antibodies Recognizing the Extreme N-terminus of PrP^C

Figures & data

Figure 1 N-terminal bovine (A) and human (B) chimeric peptide used as immunogens for the production of YWH antibodies.

Figure 2 Cross reactions of YWH1 and YWH2 using various peptides.

Figure 3 Measurement of disease-associated PrP in histology-confirmed scrapie positive and negative rostral medulla using Western blot and DELFIA®.

Figure 4 Measurement of disease-associated PrP in vCJD-spiked human tonsil homogenate.

Table 1 Measurement of disease-associated PrP in histology-confirmed BSE positive and negative bovine caudal medulla.

	Negatives	Positives
Mean	0.046 ng/ml	14.22 ng/ml
St. Dev.	0.075 ng/ml	18.07 ng/ml
Range	0.1–0.253 ng/ml	0.928–80.35 ng/ml
n	200	200

Table 2 Measurement of disease-associated PrP in histology-confirmed Scrapie positive and negative ovine caudal medulla (A), rostral medulla (B), palatine tonsil (C) and retropharyngeal lymph node (D)

		Negatives	Positives
A. Caudal Medulla
	Mean	0.39 ng/ml	156.5 ng/ml
	St. Dev.	0.25 ng/ml	313.3 ng/ml
	Range	0.05 to 0.876 ng/ml	4.53 to 1156 ng/ml
	n	13	13
B. Rostral Medulla
	Mean	0.66 ng/ml	815.1 ng/ml
	St. Dev.	0.39 ng/ml	1188.4 ng/ml
	Range	0.2 to 1.75 ng/ml	7.9 to 3476.9 ng/ml
	n	18	22
C. Palatine Tonsil
	Mean	0.08 ng/ml	25.1 ng/ml
	St. Dev.	0.07 ng/ml	47.3 ng/ml
	Range	0.01 to 0.255 ng/ml	1.3 to 168.2 ng/ml
	n	18	26
D. Retropharyngeal Lymph Node
	Mean	0.03 ng/ml	5.89 ng/ml
	St. Dev.	0.02 ng/ml	7.01 ng/ml
	Range	0.01 to 0.06 ng/ml	0.63 to 30.3 ng/ml
	n	20	20