Susceptibility of cell substrates to PrP^Sc infection and safety control measures related to biological and biotherapeutical products

Figures & data

Table 1 Partial list of cell lines susceptible to PrP^Sc infection

Cell line designation	Cell line	Reference
GT1-7	Hypothalamic neuronal cell line (mouse)	62, 76
l-fibroblast	Fibroblast cell line (mouse)	17
Scn2a	Neuroblastoma cell line (mouse)	14
PC12	Pheochromocytoma cell line (rat)	17, 70, 71
SMB	Mesodermal cell line (mouse)	8, 16
ROV	Kidney epithelial cell line (rabbit) expressing ovine prion protein	92
MOV	Neuroglia cell line (mouse) expressing ovine prion protein	3
NIH/3T3	Fibroblast cell line (mouse)	93
L929	Fibroblast cell line (mouse)	93
SN56	Cholinergic septal neuronal cell line (mouse)	5
NSC	Fetal neural stem cells (mouse)	57
MPC	Adult multipotent progenitor cells (mouse)	57
C2C12	Myoblasts (mouse)	22
Neurosphere cell line	Central nervous system stem cell (mouse)	29
Neurons	Primary cells (mouse)	20
Astrocytes	Primary cells (mouse)	20
Dendritic cells	Primary cells (human)	72

Nishida N, Harris DA, Vilette D, Laude H, Frobert Y, Grassi J, Casanova D, Milhavet O, Lehmann S. Successful transmission of three mouse-adapted scrapie strains to murine neuroblastoma cell lines overexpressing wild-type mouse prion protein. J Virol 2000; 74:320 - 325

 PubMed Web of Science ®Google Scholar

Schatzl HM, Laszlo L, Holtzman DM, Tatzelt J, DeArmond SJ, Weiner RI, Mobley WC, Prusiner SB. A hypothalamic neuronal cell line persistently infected with scrapie prions exhibits apoptosis. J Virol 1997; 71:8821 - 8831

 PubMed Web of Science ®Google Scholar

Clarke MC, Millson GC. Infection of a cell line of mouse L fibroblasts with scrapie agent. Nature 1976; 261:144 - 145

 PubMed Web of Science ®Google Scholar

Butler DA, Scott MR, Bockman JM, Borchelt DR, Taraboulos A, Hsiao KK, Kingsbury DT, Prusiner SB. Scrapie-infected murine neuroblastoma cells produce protease-resistant prion proteins. J Virol 1988; 62:1558 - 1564

 PubMed Web of Science ®Google Scholar

Rubenstein R, Carp RI, Callahan SM. In vitro replication of scrapie agent in a neuronal model: infection of PC12 cells. J Gen Virol 1984; 65:2191 - 2198

 PubMed Web of Science ®Google Scholar

Rubenstein R, Deng H, Scalici CL, Papini MC. Alterations in neurotransmitter-related enzyme activity in scrapie-infected PC12 cells. J Gen Virol 1991; 72:1279 - 1285

 PubMed Web of Science ®Google Scholar

Birkett CR, Hennion RM, Bembridge DA, Clarke MC, Chree A, Bruce ME, Bostock CJ. Scrapie strains maintain biological phenotypes on propagation in a cell line in culture. EMBO J 2001; 20:3351 - 3358

 PubMed Web of Science ®Google Scholar

Clarke MC, Haig DA. Evidence for the multiplication of scrapie agent in cell culture. Nature 1970; 225:100 - 101

 PubMed Web of Science ®Google Scholar

Vilette D, Andreoletti O, Archer F, Madelaine MF, Vilotte JL, Lehmann S, Laude H. Ex vivo propagation of infectious sheep scrapie agent in heterologous epithelial cells expressing ovine prion protein. Proc Natl Acad Sci USA 2001; 98:4055 - 4059

 PubMed Web of Science ®Google Scholar

Archer F, Bachelin C, Andreoletti O, Besnard N, Perrot G, Langevin C, Le DA, Vilette D, Baron-Van EA, Vilotte JL, Laude H. Cultured peripheral neuroglial cells are highly permissive to sheep prion infection. J Virol 2004; 78:482 - 490

 PubMed Web of Science ®Google Scholar

Vorberg I, Raines A, Story B, Priola SA. Susceptibility of common fibroblast cell lines to transmissible spongiform encephalopathy agents. J Infect Dis 2004; 189:431 - 439

 Google Scholar

Baron GS, Magalhaes AC, Prado MA, Caughey B. Mouse-adapted scrapie infection of SN56 cells: greater efficiency with microsome-associated versus purified PrP-res. J Virol 2006; 80:2106 - 2117

 PubMed Web of Science ®Google Scholar

Milhavet O, Casanova D, Chevallier N, McKay RD, Lehmann S. Neural stem cell model for prion propagation. Stem Cells 2006; 24:2284 - 2291

 PubMed Web of Science ®Google Scholar

Dlakic WM, Grigg E, Bessen RA. Prion infection of muscle cells in vitro. J Virol 2007; 81:4615 - 4624

 PubMed Web of Science ®Google Scholar

Giri RK, Young R, Pitstick R, DeArmond SJ, Prusiner SB, Carlson GA. Prion infection of mouse neurospheres. Proc Natl Acad Sci USA 2006; 103:3875 - 3880

 PubMed Web of Science ®Google Scholar

Cronier S, Laude H, Peyrin JM. Prions can infect primary cultured neurons and astrocytes and promote neuronal cell death. Proc Natl Acad Sci USA 2004; 101:12271 - 12276

 PubMed Web of Science ®Google Scholar

Rybner-Barnier C, Jacquemot C, Cuche C, Dore G, Majlessi L, Gabellec MM, Moris A, Schwartz O, Di SJ, Cumano A, Leclerc C, Lazarini F. Processing of the bovine spongiform encephalopathy-specific prion protein by dendritic cells. J Virol 2006; 80:4656 - 4663

 PubMed Web of Science ®Google Scholar