Figures & data
Figure 1. Sensitive detection of CWD prions by RT-QuIC. (A) RT-QuIC reactions using cervid PrP as a substrate were seeded with serial dilutions of brain homogenate (10%) derived from a CWD-infected (CWD+) or non-infected (CWD-) mule deer. (B) Ten % CWD infected brain homogenate was diluted 10−3 or 10−4 with urine from a CWD-negative deer. Serial dilutions thereof were used to seed quadruplicate RT-QuIC reactions using cervid recombinant PrP as a substrate. The y-axis shows the mean ThT fluorescence. The x-axis depicts the reaction time.
Figure 2. Detection of CWD prions in urine of pre-clinical deer. Urine of orally infected animals (W804 = mule deer; W1004 = white-tailed deer) collected 5, 13 and 16 mo post infection, respectively, was diluted 20-fold in RT-QuIC buffer and was then used to seed RT-QuIC reactions as described using cervid PrP as a substrate. Urine of non-infected animals served as a negative control.
Figure 3. CWD prions are detectable in feces by RT-QuIC. Extracts of white-tailed deer (W1004) feces collected at 20 or 30 mo post oral inoculation were seeded either undiluted or in a 2 × 10−1 dilution into quadruplicate reactions. Fecal extracts from non-infected deer served as a negative control.
