32k Da protein improve ovariectomy-induced bone loss in rats

Figures & data

Table 1. Effect of 32Kda protein on the weight of body and uterus

Group	SHAM	OVX	E2	32KP 50	32KP 100	32KP 150
Body weights (g)
Initial	202 + 2.5	202 + 3.0	201 + 3.7	202 + 2.9	203 + 1.9	202 + 3.1
Final	283 + 7.0	290 + 6.6##	301 + 6.2	243 + 4.6*	251 + 3.8**	248 + 3.9**
Uterus (g)	0.6100 + 0.19	0.0750 + 0.012##	0.1786 + 0.02*	0.1856 + 0.02	0.1957 + 0.02	0.2014 + 0.02

Values are mean ± SEM *p < 0.05, **p < 0.01 vs.OVX; #p < 0.05, ##p < 0.01 vs. SHAM as evaluated by ANCOVA.

Table 2. Effects of 16 weeks treatment with 32KP or E2 on biochemical parameters in serum and urine of ovariectomized (OVX) rats

Parameter	SHAM	OVX	E2	32KP 50	32KP 100	32KP 150
S-Ca (mmol/L)	3.54 ± 0.49	3.81 ± 0.31	3.28 ± 0.25	3.40 ± 0.42	3.48 ± 0.32	3.95 ± 0.38
S-P (mmol/L)	1.32 ± 0.19	1.87 ± 0.25	1.64 ± 0.20	1.78 ± 0.34	1.89 ± 0.35	1.95 ± 0.37
U-Ca/Cr (mmol/L/mmol/L)	0.65 ± 0.15	0.49 ± 0.02**	0.28 ± 0.01##	0.46 ± 0.04##	0.45 ± 0.04##	0.48 ± 0.03##
U-P/Cr (mmol/L/mmol/L)	3.47 ± 0.29	4.38 ± 0.47**	4.21 ± 0.52#	4.40 ± 0.68	4.52 ± 0.0.62	3.98 ± 0.51#
ALP (U/L)	180.45 ± 21.22	230.45 ± 25.40**	175.40 ± 52.45##	229.31 ± 41.24	237.92 ± 18.41##	203.48 ± 16.25##
OC (nmol/L)	9.78 ± 0.73	12.37 ± 0.98**	9.46 ± 0.85##	9.53 ± 0.89	12.68 ± 0.86##	12.44 ± 0.73##
DPD (nmol/L)	56.47 ± 4.25	96.74 ± 7.14**	67.50 ± 6.87##	68.988 ± 7.87	84.55 ± 4.76##	75.52 ± 8.05#

Values are mean ± SEM *p < 0.05, **p < 0.01 vs.OVX; #p < 0.05, ##p < 0.01 vs. SHAM as evaluated by ANCOVA.

Figure 1. Effects of 16-weeks treatment with 32KP or E2 on (A) the total bone mineral content (t-BMC) and (B) bone mineral density (t-BMD) in right femur of ovariectomized (OVX) Rats by DEXA. Values are mean ± SEM *p < 0.05, **p < 0.01 vs.OVX; #p < 0.05, ##p < 0.01 vs. SHAM as evaluated by ANCOVA.

Table 3. Effects of 16 weeks treatment with 32KP or E2 on bone biomechanical paremeters in the femoral diaphysis of ovariectomized (OVX) rats

Parameters	SHAM	OVX	E2	32KP 50	32KP 100	32KP 150
Maximum load (N)	103.56 ± 6.18	103.56 ± 6.19*	100.56 ± 9.38##	106.56 ± 10.05#	98.76 ± 6.92##	104.18 ± 9.16#
Stiffness (N/mm)	170.36 ± 19.19	152.30 ± 16.85**	159.56 ± 16.27#	154.56 ± 17.25	154.56 ± 21.03##	146.56 ± 27.15##
Energy (N/mm)	39.55 ± 3.25	33.75 ± 5.19	33.78 ± 5.39#	33.41 ± 2.18##	29.61 ± 4.22##	34.84 ± 8.07#
Maximum stress (Mpa)	193.58 ± 6.91	151.87 ± 12.38*	178.56 ± 6.24##	103.56 ± 6.21	158.27 ± 27.30##	189.56 ± 30.23#
Yong'Modulus (Mpa)	6752.46 ± 1096.27	4750.83 ± 695.25**	5897.85 ± 978.20##	5754.56 ± 1024.29##	6028.37 ± 8016.13##	6521.03 ± 2315.85##

Values are mean ± SEM *p < 0.05, **p < 0.01 vs.OVX; #p < 0.05, ##p < 0.01 vs. SHAM as evaluated by ANCOVA.

Figure 2. Hematoxylin-eosin staining of OVX, SHAM, E2 and different dose 32KP (A–F). The marrow cavity of the femurs was observed by light microscopy (X40).