Figures & data
Figure 1. Prion related synthetic peptide fragments and the de novo designed peptide for the structural conversion, designated HPPSH. Each Cys-residue was replaced by a Ser-residue to avoid disulfide bond formation. Hence hPrP170–175 (SNQNNF) formed parallel β-sheet [PDB ID: 2OL9] and hPrP180–195 (VNITIKQHTVTTTTKG) formed α-helix [PDB ID: 2IV4].
![Figure 1. Prion related synthetic peptide fragments and the de novo designed peptide for the structural conversion, designated HPPSH. Each Cys-residue was replaced by a Ser-residue to avoid disulfide bond formation. Hence hPrP170–175 (SNQNNF) formed parallel β-sheet [PDB ID: 2OL9] and hPrP180–195 (VNITIKQHTVTTTTKG) formed α-helix [PDB ID: 2IV4].](/cms/asset/9fefd712-4ed6-4a82-8b76-1064fd32919e/kprn_a_10927961_f0001.gif)
Figure 2. Scheme for the preparation of peptide library derived bovine brain. Abbreviations used are CF, centrifugation (10 000 rpm, 4 °C); PPT, precipitate; SCA, structural conversion assay. Chromatogram is a tryptic digests and fractions eluted at 7–8 and 15–16 min (arrow) exhibited significance in SCA.
