Figures & data
Figure 1 96-well PrPSc immunoassay controls. (A) 96-well immunoassay and (B) quantification of brain homogenate from proteinase K digested uninfected (UN) or HY TME-agent infected (HY TME) hamsters with (+) and without (−) H2O2 in MeOH, primary antibody or secondary antibody to indicate the specificity of PrPSc immunodetection. Relative PrPSc intensity is based on well 16.
![Figure 1 96-well PrPSc immunoassay controls. (A) 96-well immunoassay and (B) quantification of brain homogenate from proteinase K digested uninfected (UN) or HY TME-agent infected (HY TME) hamsters with (+) and without (−) H2O2 in MeOH, primary antibody or secondary antibody to indicate the specificity of PrPSc immunodetection. Relative PrPSc intensity is based on well 16.](/cms/asset/6deb2a59-ea63-47da-a4a4-9108662f8bfd/kprn_a_10908442_f0001.gif)
Figure 2 Limits of PrP detection using western blot analysis. Western blot analysis of two-fold serial dilutions of brain homogenates treated with (+) and without (−) proteinase K (PK) from uninfected hamsters (UN, A) or hamsters infected with the HY TME (HY) or DY TME (DY) agents (B and C, respectively).
![Figure 2 Limits of PrP detection using western blot analysis. Western blot analysis of two-fold serial dilutions of brain homogenates treated with (+) and without (−) proteinase K (PK) from uninfected hamsters (UN, A) or hamsters infected with the HY TME (HY) or DY TME (DY) agents (B and C, respectively).](/cms/asset/ced9f2ad-da32-4c6e-9c76-42b84fd54773/kprn_a_10908442_f0002.gif)
Figure 3 Limits of PrP detection using 96-well immunoassay. 96 well immunoassay of two-fold serial dilutions of brain homogenate from uninfected (UN), HY TME agent (HY) or DY TME agent (DY) infected hamsters. Samples were either proteinase K (PK) digested (+) or were left untreated (−).
![Figure 3 Limits of PrP detection using 96-well immunoassay. 96 well immunoassay of two-fold serial dilutions of brain homogenate from uninfected (UN), HY TME agent (HY) or DY TME agent (DY) infected hamsters. Samples were either proteinase K (PK) digested (+) or were left untreated (−).](/cms/asset/017d83dd-a27a-4a75-b593-e5c3f766d41d/kprn_a_10908442_f0003.gif)
Figure 4 Comparison of PrPSc detection limits by western blot and 96-well immunoassay. Quantification of PrPSc abundance of two-fold serial dilutions of uninfected (UN), HY TME-infected (HY) or DY TME-infected (DY) proteinase K digested brain material by either western blot (open bars) or 96-well immunoassay (solid bars). Represented data is the average and standard error of the mean of three independent experiments.
![Figure 4 Comparison of PrPSc detection limits by western blot and 96-well immunoassay. Quantification of PrPSc abundance of two-fold serial dilutions of uninfected (UN), HY TME-infected (HY) or DY TME-infected (DY) proteinase K digested brain material by either western blot (open bars) or 96-well immunoassay (solid bars). Represented data is the average and standard error of the mean of three independent experiments.](/cms/asset/bc250563-d038-42d7-a1d6-65a982948651/kprn_a_10908442_f0004.gif)