Prion protein function and the disturbance of early embryonic development in zebrafish

Figures & data

Figure 1 PrP-2 gene knockdown induced differentially expressed genes involved in neurogenesis and developmental processes in 24 hpf zebrafish embryos injected with PrP-2 morpholino. Red color indicates up and green downregulation of the genes indicated. The figure was generated by Ingenuity Pathway Analysis program (IPA; www.ingenuity.com), which clusters significantly differentially expressed genes and generates a pathway and biological function analysis. The symbols for different gene products involved are presented in the top part.

Figure 2 PrP-2 mRNA splice isoforms: At least two isoforms of prnprs3 (PrP-2) mRNA are described in zebrafish; (A) A 2 exon transcript (RefSeq: NM_001013298.1; UCSC Genome Browser on Zebrafish Jul 2010) and (B) A 3 exon transcript (ENSDART00000116357, Zv9 Ensembl release 61-Feb 2011). The latter discloses an extra intron in the C-terminus of the open reading frame leading to a frame shift, which causes the C-terminal end to switch from being hydrophobic to more hydrophilic. The figure was created using CLC Main Workbench (CLC bio, Aarhus, Denmark).

Table 1 Gene accession numbers, gene symbols, names, molecule type and fold change for differentially expressed genes involved in neurogenesis and developmental processes in 24 hpf zebrafish embryos injected with PrP-2 morpholino

Genbank Accession Number	Mammalian Gene Symbol	Entrez Gene Name	Molecule type	Fold change
BI430331	ABCC5	ATP-binding cassette, sub-family C (CFTR/MRP), member 5	Transporter	2,74
BC076071	ACHE	acetylcholinesterase	Enzyme	−1,65
NM_131342	BMP4	bone morphogenetic protein 4	Growth factor	1,37
BM025541	COL18A1	collagen, type XVIII, alpha1	Unknown	1,32
AF052249	FOXD3	forkhead box D3	Transcription regulator	1,36
AW777320	GFAP	glial fibrillary acidic protein	Unknown	−1,39
Y08426	IHH	Indian hedgehog	Enzyme	1,88
BM182277	MMP2	matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase)	Peptidase	1,61
AI883922	PHGDH	phosphoglycerate dehydrogenase	Enzyme	−1,56
BM095161	PTP4A3	protein tyrosine phosphatase type IVA, member 3	Phosphatase	1,39
BI865912	S1PR1	sphingosine-1-phosphate receptor 1	G-protein coupled receptor	−1,85
U30710	SHH	sonic hedgehog	Peptidase	−1,41
U60804	TP53	tumor protein p53	Transcription regulator	3,36