Characterization of potato plants with reduced StSYR1 expression

Figures & data

Figure 1. Model of StSYR1 function. (A) Mechanism of StSYR1-function in wild type potato plants. (B) Deregulated StSYR1-dependent mechanisms in StSYR1-RNAi plants. For details see the main text.

Figure 2.StSYR1-RNAi lines are impaired in tuber development. Plants were cultivated in the green house in five liter pots under standard conditions. After about three months, tubers had matured and were harvested. (A) Representative tubers of wild type plants and one StSYR1-RNAi line (i1) are shown. (B) The amounts of tubers per plant, as well as the tuber weights, totally per plant and individually are plotted. “Control,” wild type and empty vector-transformed plants; “StSYR1-RNAi,” 11 independent StSYR1-RNAi construct-transformed lines. Statistically significant differences are depicted (GraphPad Prism 5, Mann-Whitney-Test). The experiment was performed three times with similar results.

Figure 3. Accumulation of jasmonates is not altered in StSYR1-RNAi plants. Plants were grown for three weeks in a phytochamber with 16 h light (140 µE) at 60% humidity and 20°C. Leaf material of healthy plants was sampled and used for the extraction of jasmonates. Concentrations of 12-oxo-phytodienoic acid [OPDA; (A), JA (B) and JA-isoleucine (JA-Ile; (C)] are plotted. Control, wild type and empty vector-transformed plants; StSYR1-RNAi, StSYR1-RNAi construct-transformed lines; fw, fresh weight. The experiment was done twice with similar results with at least ten independent StSYR1-RNAi lines. No statistically significant differences between control and StSYR1-RNAi lines were measured (ns, not significant; GraphPad Prism 5, Mann-Whitney-Test).

Figure 4. Differential secretion of metabolites in StSYR1-RNAi cell cultures. Cell cultures were grown for four days in the dark with continuous shaking at 20°C. (A) Individual cultures were elicited with 10 nM Pep-13 and harvested by centrifugation at the time points indicated. Total RNA was isolated and used for northern blot hybridization with a radioactively labeled StSYR1-specific probe. Ethidium bromide-stained gels are depicted to show equal loading. EV, empty vector-transformed cell culture; B4, C1, independent StSYR1-RNAi cell cultures; hpt, hours post treatment. (B and C) Cell cultures were elicited with 10 nM Pep-13, media were harvested 24 h post elicitation by filtration and used for LC-MS measurements. Two differentially occurring peaks are plotted. Experiments were performed three times with similar results.