From the Notch Signaling Pathway to Ribosome Biogenesis

Figures & data

Figure 1 cDNA microarray analysis of ribosomal proteins expression following fertilization in S. chacoense ovules. (A) Gene expression changes observed using nonparametric Anova testing (Kruskal- Wallis t-test), along with a Benjamini and Hochberg multiple testing correction algorithm of all the ribosomal protein genes available in our 7K microarray. (B) Cluster analysis of the differentially expressed ESTs corresponding to the ribosomal proteins in Table 1. The clones were classified based on the similarity of their expression profiles using a Self organization map (SOM) clustering. Three distinct SOM profiles (Group 1 to 3) can be distinguished. RNA extraction, probe preparation, cDNA array hybridization and data analysis were performed as described previously.27

Table 1 Ribosomal proteins clustering with the self organizing map (SOM) groups as determined by cDNA microarray analysis

Group 1		Group 2		Group 3
SSU	LSU	SSU	LSU	SSU	LSU
S3	L4	S8	L1	S16	L5
S4	L6	S9	L2	S17	L7a
S5	L11	S21	L8	S18	L9
S6	L21	S28	L10a	S23	L13
S10	L24		L12	S26	L13a
S11	L32		L34	S27	L14
S13	L36		L37	S30	L17
S14	L38		L37a		L18
S15	L41				L22
S19				RAP*	L23a
S20				P0	L26
S25				P1	L28
S29				P2	L29
S29a				P3	L30
				Sa (P40)	L31
					L33
					L35

* RAP, ribosomal associated proteins. Abbreviations: SSU, ribosome small subunit; LSU, ribosome large subunit.

Gray-Mitsumune M, O'Brien M, Bertrand C, Tebbji F, Nantel A, Matton DP. Loss of ovule identity induced by overexpression of the fertilization-related kinase 2 (ScFRK2), a MAPKKK from Solanum chacoense. J Exp Bot 2006; 57:4171 - 4187

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